Audit: QRS - Apigenin for Health & Longevity

Audit conducted on 22/09/2026 16:03 using AI4L / Opus 5

Iterations

Summary

Items Count
Total 94
Passed 88
Failed 0
N/A 6
Pass Rate 100.00%
  • Total = Passed + Failed + N/A
  • Pass Rate = Passed / (Passed + Failed) × 100
  • N/A items are excluded from the pass rate calculation

1. General Rules

# Description Result Comments
1.1 Every claim, magnitude, label, recommendation, and statement in the QRS is literally supported by content in the source ER. 🟢 Every span traced to source: at-a-glance to ER Conclusion (L492-496); protocol cells to ER Therapeutic Protocol (L364, L366, L376); time cells to ER L175, L419, L359; benefits/risks to ER tier headings; gates to ER L313-340; monitoring table to ER L449-457; cadence to ER L447; qualitative items to ER L461-466.
1.2 Where the ER uses cautious phrasing (“not formally studied”, “None documented in human trials to date”, “theoretical concern”, “data are limited”), the QRS uses the same phrasing. 🟢 “No established timeline” and “No human study has measured one” mirror ER L419; “if present” retained for the sedative effect; “Speculative” and “theoretical platelet effect” carried through from ER L457.
1.3 The QRS never strengthens an ER claim (e.g., “not formally studied” → “not required”) or softens one (e.g., “do not use during pregnancy” → “use with caution during pregnancy”). 🟢 Contraindications stay absolute (pregnancy, transplant, Child-Pugh B/C) as in ER L335-340; the sleep claim is not upgraded — the QRS repeats the null insomnia result alongside the sedative timing.
1.4 The QRS does not relabel an ER fact under a different decision category. A “Benefit-Modifying Factor” from ER section is not surfaced as a “Caution”; a “Risk-Modifying Factor” is not surfaced as a “Side Effect”; etc. 🟢 Contraindications come only from the ER “Populations who should avoid Apigenin” list; Key Interactions only from the ER interaction bullets; no Benefit- or Risk-Modifying Factor is surfaced as a gate.
1.5 PubMed IDs, study citations, expert names, clinical trial identifiers (NCT*), and brand names appear in the QRS only if they appear in the source ER for the same fact. 🟢 The QRS carries no PMIDs, no NCT identifiers, no author names and no brand names; the sepsis pilot trial is referenced generically, as the ER does at L364.
1.6 The QRS does not introduce new attributions. 🟢 No attributions of any kind appear on the sheet.

2. Focus, Tone & Audience

# Description Result Comments
2.1 The QRS follows the tone of the ER, which is determined by the ER’s own language, phrasing, and framing. 🟢 Matches the ER’s measured, sceptical register — “plausible, inexpensive, and unproven in either direction” (ER L496) becomes “Cheap, plausible, and unproven in either direction”.
2.2 The tone of the QRS is simultaneously expert, accessible, objective, and data-driven, but also empowering and encouraging 🟢 Presents the evidence gap plainly while giving the reader concrete, actionable protocol, monitoring and qualitative-assessment anchors.
2.3 The QRS reads as a trusted, knowledgeable guide rather than a prescriptive doctor 🟢 Declarative throughout; no imperative instructions.
2.4 The QRS avoids language that implies medical or clinical advice 🟢 Monitoring and cadence are stated as what the ER describes, not as orders.
2.5 The QRS “presents information” instead of “providing guidance”, “recommending”, or “advising” 🟢 No “recommend”, “advise” or “should” in the QRS’s own voice; e.g. “a 12-week reassessment point is where discontinuation is considered”.
2.6 The QRS never addresses “the reader” directly — it presents evidence, not guidance 🟢 No second-person pronouns anywhere in the file.
2.7 The QRS is written in plain language, avoiding unnecessary medical jargon 🟢 Plain phrasing used wherever the ER allows (“cellular fuel molecule”, “worn-out-cell secretome” from the ER heading); remaining technical terms are drug-class names the gate cannot function without.
2.8 Information is presented in a concise and very compact manner 🟢 Every item is a bare fact; ER elaborations, magnitudes and citations are dropped.
2.9 It DOES NOT address the reader directly 🟢 Confirmed — no direct address anywhere.
2.10 The target audience is health- and longevity-oriented adults who are risk-aware, proactive, and actively seeking to optimize health or apply the intervention under review. 🟢 Protocol, biomarker panel and qualitative self-assessment list all assume a proactive self-experimenter.
2.11 The target audience is willing to employ lifestyle and behavioral changes as well as follow protocols that may be inconvenient, costly, or require effort. 🟢 Monthly home blood-pressure and weight checks plus a seven-marker blood panel presuppose that willingness.
2.12 The document is NOT written for the general population, who are unwilling to employ lifestyle and behavioral changes or follow protocols that may be inconvenient, costly, or require effort. 🟢 Content level and the narrow-therapeutic-index interaction detail are well above general-population framing.
2.13 Framing, takeaways, and risk/benefit weighting throughout the document reflect this audience, including where an intervention’s signal for the average person differs from its signal for this audience. 🟢 The sheet foregrounds that the longevity rationale is unmeasured in humans while the sedative rationale drives the actual protocol — the distinction that matters to this audience.
2.14 The document’s own voice frames usage in longevity terms, not “anti-aging” (e.g., “anti-aging clinics”, “anti-aging community”, “anti-aging medicine”). Proper names that contain “anti-aging” (e.g., “American Academy of Anti-Aging Medicine”) are quoted verbatim. 🟢 The string “anti-aging” does not occur; the sheet uses “ageing-related effect” and “falls with age”.
2.15 The document’s own voice uses formal clinical and scientific terminology, not colloquial or consumer-grade language (e.g., “oral medication” not “pill(s)”; “oral” / “administered orally” not “taken by mouth” / “given by mouth”; “injection” not “shot”; “adverse event” not “bad reaction”). This holds on EVERY surface, including the QRS lede — the stricter plain-language bar (see 2.7) does not license lay phrasing for route of administration. Direct quotes from sources are exempt. 🟢 “capsule”, “dose”, “sedation”, “allergic reactions” used; no “pill”, “shot”, “taken by mouth” or “bad reaction”. The lede’s “evening capsule” matches the ER’s own wording at L492.

3. Template Integrity

# Description Result Comments
3.1 The following labels and headings on the QRS are fixed and not modified: Card and section headings: “Protocol”, “Time to effect”, “Benefits”, “Risk & Side Effects”, “Monitoring”, “Qualitative Assessment”; Gate headings: “Contraindications”, “Key Interactions”; Tier labels: “High”, “Medium”, “Low”, “Speculative”; Table column headers in Monitoring: “Marker”, “Target”, “Why” 🟢 All fourteen fixed strings verified verbatim against the template (QRS L446, L492, L543, L575, L594, L630, L657, L661-663, L781, and the four tier labels).
3.2 All “<span data-qrs-var=”NAME”>…</span>” from the [qrs_template] are present in the the QRS. 🟢 Set comparison of all data-qrs-var names shows zero template variables missing; the only additions are the expected expansions of marker_#* into marker_1..7 and qualitative_item# into 1..6.
3.3 Spans that are not addressed in a checklist item are left unchanged 🟢 The three website=”…” spans (evidence_review, audit, full_review), the disclaimer and the entire <style> block are byte-identical to the template.

4. Formatting

# Description Result Comments
4.1 When the source ER section is empty, the QRS uses the ER’s own empty-state phrasing verbatim. Typical phrasings are “None documented in human trials to date” and “Not formally studied” N/A No source ER section is empty. The three unpopulated tiers (benefits High, risks High and Medium) carry explanatory ER text and are governed by 12.5/13.5, which explicitly forbid empty-state phrasing.
4.2 Where the ER presents a bulleted item as “Label: content”, the QRS uses the ER’s bold label verbatim as the cell or row label. 🟢 Protocol labels “Standard dose”, “Timing” and “Single versus split dosing” are the ER’s bold labels verbatim (ER L364, L366, L376); the interaction gate carries the ER’s bold labels including their example-drug parentheses.
4.3 Labels are not paraphrased, abbreviated, or invented. 🟢 No invented label appears; monitoring row labels are the ER biomarker names verbatim.
4.4 The QRS DOES NOT use emoji indicators (no 🟩, 🟥, 🟨, etc.). Color and emphasis are conveyed through CSS and bold labels. 🟢 Codepoint scan returns no emoji; the ER’s 🟩/🟥/🟨 tier markers and the ⚠️ Conflicted flags were correctly dropped.
4.5 The QRS is designed to render on one A4 page. Any section that has more content in the ER than fits the per-section budget is condensed by the LLM, not extended onto a second page. 🟢 Every section is condensed to bare facts: benefit and risk tiers collapsed into single semicolon-separated lines, gate items stripped of their rationale clauses, monitoring “Why” cells reduced to the ER’s short reason, and the cadence paragraph compressed from ER L447.

5. Metadata

# Description Result Comments
5.1 The metadata is placed inside a single HTML comment that is the first element after “<!doctype html>” and before any other comment, head, or body content. 🟢 QRS L2-14: the metadata comment immediately follows the doctype and precedes the template comment at L16.
5.2 Inside that HTML comment the YAML block is delimited by a line “—” opening and a line “—” closing. Text before the opening “—” is permitted but is not parsed as YAML. 🟢 Opening “—” at L3, closing “—” at L13; the preamble text sits on L2.
5.3 The metadata is not visible in any rendered view of the QRS and is not surfaced by any other element on the sheet. 🟢 Enclosed entirely in an HTML comment; no metadata value is repeated in the head or body.
5.4 All frontmatter values are trimmed: no leading or trailing whitespace, no surrounding quotes unless the value contains a colon, bracket, or leading special character that requires YAML quoting. 🟢 Only duration: "00:03" is quoted, and it contains a colon; all other values are bare and trimmed.
5.5 The filename of the source ER is stated as “er_filename: [er_filename]” 🟢 L4: er_filename: apigenin_2026-0906-0456_Opus_ER.md, matching the ER’s own filename field at ER L17.
5.6 Version of the QRS.md file used to create the document is stated as “qrs_prompt_version: [Version of QRS.md]” 🟢 L5: qrs_prompt_version: 26.9.22, matching the QRS.md version badge.
5.7 Creation date and time of the document is stated as “qrs_creation_date: [YYYY-MMDD-HHMM]” (e.g., 2026-0501-1430) 🟢 L6: qrs_creation_date: 2026-0922-1556 — correct format.
5.8 The nickname of the AI used to create the document is stated as “qrs_creator_ai_nickname: [qrs_creator_ai_nickname]” 🟢 L7: qrs_creator_ai_nickname: Opus.
5.9 The nickname of the AI is just a single word model name without version, etc. (e.g., Opus, Sonnet, Grok, Gemini, ChatGPT) 🟢 “Opus” — single word, no version.
5.10 The full name of the AI used to create the document is stated as “qrs_creator_ai_fullname: [qrs_creator_ai_fullname]” 🟢 L8: qrs_creator_ai_fullname: Opus 5.
5.11 The full name of the AI consists of the [qrs_creator_ai_nickname] and the model version number and no additional qualifier (e.g., Opus 4.6, Sonnet 3.2, Grok 4.5, Gemini 3.1, ChatGPT 5.4) 🟢 “Opus 5” — nickname plus version, no qualifier.
5.12 The filename of the document is stated as “qrs_filename: [filename of this document]” 🟢 L9: qrs_filename: apigenin_2026-0906-0456_Opus_QRS.html, matching the file on disk.
5.13 All frontmatter values are trimmed: no leading or trailing whitespace, no surrounding quotes unless the value contains a colon, bracket, or leading special character that requires YAML quoting. 🟢 Re-verified across all nine keys; no stray whitespace or unnecessary quoting.

6. Page Title & Header

# Description Result Comments
6.1 [page_title] is set to the [canonical_topic] of the ER frontmatter followed by “ - Quick Reference Sheet” (e.g., “Intervention - Quick Reference Sheet”). The [canonical_topic] is HTML-entity-encoded as needed (e.g., &amp; for &) 🟢 QRS L22: “Apigenin for Health & Longevity - Quick Reference Sheet” against ER canonical_topic at L8.
6.2 [header_topic] is set to the [canonical_topic] of the ER frontmatter, with HTML entities encoded as needed (e.g., &amp; for &) 🟢 QRS L417: “Apigenin for Health & Longevity”.
6.3 [header_subline_date] is set to [qrs_creation_date reformatted as MM/DD/YYYY] 🟢 QRS L421: “09/22/2026” from qrs_creation_date 2026-0922-1556.
6.4 [header_subline_model] is set to [qrs_creator_ai_fullname] 🟢 QRS L425: “Opus 5”.
6.5 No additional header content appears: no badge, version stamp, AKA / alternate names line, source-AI attribution, audit date, or QRS variant marker. 🟢 Header holds only the title and the standard subline; the ER’s “Also known as” line (ER L30) was correctly not carried over.

7. At-A-Glance Section

# Description Result Comments
7.1 [at_a_glance] opens by saying what the intervention is — its kind (e.g., dietary supplement, prescription medication, peptide, plant, procedure, practice) — and what it is used for, in plain language, before any verdict on the evidence 🟢 Opens “Apigenin is a plant compound from chamomile, parsley and celery, sold as an evening capsule on two promises…” — kind and purpose stated before any evidence verdict.
7.2 [at_a_glance] is dense, execution-oriented summary of the ER Conclusion section 🟢 Condenses ER L492 and L496 into five clauses covering identity, the two claims, the human-evidence gap and the net verdict.
7.3 [at_a_glance] is no longer than 70 words 🟢 65 words.
7.4 Every fact in [at_a_glance] is supported by a distinct passage in the ER. 🟢 Plant sources and evening capsule (ER L492); the two promises (ER L37, L492); no purified-apigenin human outcome (ER L492); cells/animals/chamomile extract (ER L492); cheap, plausible, unproven (ER L487, L496).
7.5 It DOES NOT use acronyms or technical classifications that require specialist knowledge, uses plain-language terms instead 🟢 No acronyms; NAD⁺ is rendered as “a cellular fuel molecule that falls with age” and GABA as “calms the nervous system”.
7.6 It DOES NOT cite specific trials (names, years, sample sizes, p-values) 🟢 No trial name, year, sample size or p-value appears.
7.7 It DOES NOT cite effect sizes, relative risks, or statistical results 🟢 No numeric result of any kind.

8. Contraindications

# Description Result Comments
8.1 The section is derived from the ER Key Interactions & Contraindications section 🟢 All six items come from the “Populations who should avoid Apigenin” list at ER L333-340.
8.2 [stop_items] represent the Contraindications from the ER 🟢 Six ER populations, six QRS items, one-to-one and in ER order.
8.3 Individual [stop_items] are formatted as <li></li> 🟢 QRS L578-589: six discrete <li> elements inside the stop_items span.
8.4 Items are as concise as possible. No trailing explanations, no elaborations, no mechanistic rationale, no attributions, no citations, no study details. No content after an em-dash, en-dash, or hyphen-dash (e.g., “— dose reduction required”, “— reduced efficacy”) — these trailing clauses are stripped. Just the key fact. 🟢 The ER’s trailing rationales are all stripped: “— safety data are absent…”, “where a shift in drug exposure risks rejection or toxicity”, “in whom conjugation capacity is materially reduced”, “following the standard supplement washout…”.
8.5 Parenthetical qualifiers from the ER bullet — time windows, severity classes, threshold values, clinical staging — ARE preserved as part of the item, kept as concise as possible (shortened or trimmed where needed to fit the one-page budget, but never dropped entirely). 🟢 “Child-Pugh Class B or C” and “within two weeks of scheduled surgery” preserved, as is the chamomile-derived-product condition; only the pure glosses (“a severity grading for chronic liver disease”, “oestrogen- or testosterone-blocking drugs”) are dropped.
8.6 When the ER uses ranking notation inside parens (e.g., “>” for severity ordering) that depends on an explanatory phrase to interpret, normalize the items to a plain comma-separated list rather than carrying through the bare symbol. 🟢 The ER uses no ranking notation in this list; nothing to normalize and no bare symbol carried through.
8.7 The section is left empty ONLY IF the ER’s Key Interactions & Contraindications section identifies no population, condition, or scenario for which the intervention should be avoided, deferred, or used only under specialist supervision. 🟢 The ER names six such populations, and the section is correspondingly populated rather than empty.
8.8 If the section is left empty, an HTML comment inside the [stop_items] span records the ER basis for the absence, naming or quoting the ER text relied on. E.g. <!– empty: ER names no population that should avoid the intervention –> N/A The section is not empty — six contraindications are listed.

9. Key Interactions

# Description Result Comments
9.1 The section is derived from the ER Key Interactions & Contraindications section 🟢 All ten items come from the interaction bullets at ER L313-331.
9.2 [caution_items] represent the Key Interactions from the ER, excluding any that are already listed as Contraindications 🟢 Ten ER interaction bullets, ten QRS items, in ER order; no contraindicated population is duplicated here.
9.3 Individual [caution_items] are formatted as <li></li> 🟢 QRS L597-620: ten discrete <li> elements inside the caution_items span.
9.4 Items are as concise as possible. No trailing explanations, no elaborations, no mechanistic rationale, no attributions, no citations, no study details. No content after an em-dash, en-dash, or hyphen-dash (e.g., “— dose reduction required”, “— reduced efficacy”) — these trailing clauses are stripped. Just the key fact. 🟢 Every ER severity word and mitigation sentence (“Caution. Additive sedation…”, “Monitor. Quercetin also inhibits CD38…”) is stripped; only the agent class remains.
9.5 Parenthetical qualifiers from the ER bullet — example drug lists, time windows, severity classes, threshold values, clinical staging — ARE preserved as part of the item, kept as concise as possible (shortened or trimmed where needed to fit the one-page budget, but never dropped entirely). 🟢 All six ER example-drug lists are preserved intact; only the explanatory gloss “(a pump that expels drugs from cells)” at ER L317 is dropped, and the ER L331 parenthetical is carried as the item itself.
9.6 When the ER uses ranking notation inside parens (e.g., “>” for severity ordering) that depends on an explanatory phrase to interpret, normalize the items to a plain comma-separated list rather than carrying through the bare symbol. 🟢 The ER uses plain comma-separated drug lists with no ranking symbols; the QRS carries them as comma-separated lists.
9.7 The section is left empty ONLY IF the ER’s Key Interactions & Contraindications section identifies no interaction, additive effect, or exposure that changes how the intervention is used. 🟢 The ER names ten interactions, and the section is correspondingly populated rather than empty.
9.8 If the section is left empty, an HTML comment inside the [caution_items] span records the ER basis for the absence, naming or quoting the ER text relied on. E.g. <!– empty: ER names no interaction that changes how the intervention is used –> N/A The section is not empty — ten key interactions are listed.

10. Protocol

# Description Result Comments
10.1 The section is derived from the ER Protocol section 🟢 All three cells come from the ER “Therapeutic Protocol” section at L362-386.
10.2 The three sets of [action] items cover the three most important actionable implementation aspects from the ER Protocol section 🟢 Dose, timing and single-versus-split dosing are the three executable decisions; the remaining ER bullets are competing positions, attributions, pharmacokinetics or “no guidance exists” statements.
10.3 If less that three distinct actionable implementation aspects are mentioned in the ER the unused sets are left empty and made invisible, not filled with placeholder text or empty-state phrasing. N/A The ER mentions more than three actionable aspects, so all three sets are used and none is left unfilled.
10.4 All used [action_#label], [action#value], [action#_sub] items are filled with meaningful content derived from the ER Protocol section. 🟢 “Standard dose / 50 mg once daily” (ER L364), “Timing / 30–60 min before bed” (ER L366), “Single versus split dosing / Single evening dose” (ER L376); each sub condenses the matching ER sentence.

11. Time to Effect

# Description Result Comments
11.1 The three sets of [time] items cover the three most important time-to-effect aspects from the ER 🟢 Anxiety onset (ER L175), the sedative effect (ER L419) and the absent metabolic/ageing timeline (ER L419) are the only time-anchored effects the ER reports.
11.2 The sets are picked and ordered by the magnitude of the related benefit 🟢 Anxiety is the ER’s strongest human signal (Low tier, ER L171-175), the sedative effect has a null randomised sleep result, and the metabolic effect is Speculative — the ordering follows that ranking.
11.3 If less that three distinct time-to-effect aspects are mentioned in the ER the unused sets are left empty and made invisible, not filled with placeholder text or empty-state phrasing. N/A Three distinct time-to-effect aspects exist in the ER, so all three sets are used.
11.4 All used [time_#label], [time#value], [time#_sub] items are filled with meaningful content derived from the ER. 🟢 “Anxiety / 8 weeks”, “Sedative effect / First night”, “Metabolic or ageing-related effect / No established timeline”; the subs draw on ER L173-175, L419 and L359.
11.5 If the ER does not provide any information on time to effect, the section is removed completely from the Protocol Panel N/A The ER does provide time-to-effect information (ER L419 “Time to effect”), so the section is correctly retained.

12. Benefits

# Description Result Comments
12.1 The section is derived from the ER Expected Benefits section 🟢 All tier content maps to the ER headings at L163-221.
12.2 Key variables are [benefits_high], [benefits_medium], [benefits_low], [benefits_speculative] 🟢 All four spans present at QRS L545, L548, L554, L562.
12.3 Items are as concise as possible. No explanations, no elaborations, no effect sizes, no qualifiers, no attributions, no citations, no study details, no mechanistic explanations, etc. Just the key fact. 🟢 Each entry is the ER heading alone; every “Magnitude:” paragraph, P value, sample size and citation is dropped.
12.4 Parenthetical content — including effect sizes, sample notes, mechanistic hints, and example studies — is stripped, NOT preserved. 🟢 “(new growths in the bowel)” is stripped from the colorectal item, as are the ⚠️ Conflicted flags on the anxiety and testosterone headings.
12.5 If no items of a specific sub-section (high, medium, low, speculative) are present the respective <SPAN> is set to “display=none”, not filled with “None documented in human trials to date” or similar empty-state phrasing. 🟢 ER L159 records no High-tier benefit; QRS L545 sets benefits_high to style="display: none" with no empty-state text.

13. Risks

# Description Result Comments
13.1 The section is derived from the ER Potential Risks & Side Effects section 🟢 All tier content maps to the ER headings at L255-289.
13.2 Key variables are [risks_high], [risks_medium], [risks_low], [risks_speculative] 🟢 All four spans present at QRS L632, L635, L638, L645.
13.3 Items are as concise as possible. No explanations, no elaborations, no effect sizes, no qualifiers, no attributions, no citations, no study details, no mechanistic explanations, etc. Just the key fact. 🟢 Each entry is the ER heading alone; the “Magnitude:” paragraphs and all PMIDs are dropped.
13.4 Parenthetical content — including frequencies, severity grades, sample notes, mechanistic hints, and example studies — is stripped, NOT preserved. 🟢 “(a whole-body allergic reaction)” and the ⚠️ Conflicted flag on the sex-hormone heading are stripped.
13.5 If no items of a specific sub-section (high, medium, low, speculative) are present the respective <SPAN> is set to “display=none”, not filled with “None documented in human trials to date” or similar empty-state phrasing. 🟢 ER L247 and L251 record no High- or Medium-tier risk; QRS L632 and L635 set both spans to style="display: none".

14. Monitoring

# Description Result Comments
14.1 The section is derived from the ER Monitoring section 🟢 Table and cadence both come from “Monitoring Protocol & Defining Success” at ER L443-457.
14.2 All measurable/quantifiable biomarkers from the Monitoring section are listed 🟢 All seven ER biomarker rows are present — hs-CRP, fasting insulin, HbA1c, ALT, TSH, total testosterone/oestradiol, and platelet count with INR — with the ER’s optimal ranges carried over verbatim.
14.3 [monitoring_cadence] is populated with the monitoring cadence/frequency derived from the ER Monitoring section. It is not left with placeholder text or empty. 🟢 QRS L771-775 condenses ER L447: monthly home blood pressure and weight, blood panel at 12 weeks then every 6–12 months, and the 2–4 week narrow-therapeutic-index drug-level check.

15. Qualitative Assessment

# Description Result Comments
15.1 The section is derived from the ER Monitoring section 🟢 Drawn from the qualitative-marker list at ER L461-466.
15.2 All subjective/qualitative biomarkers from the Monitoring section are listed 🟢 All six ER qualitative markers are carried verbatim: sleep onset, remembered awakenings, morning alertness, daytime anxiety and rumination, dream intensity, and afternoon energy and cognitive clarity.

Issues 22/09/2026 16:03

Pass rate 100.00%. No issues found.