Humanin for Health & Longevity - Quick Reference Sheet

Humanin for Health & Longevity

Created on 08/06/2026 – Quick Reference based on Evidence Review created using AI4L / Opus 5 Audit

A protein fragment from the cell's energy compartments; laboratory work shows it keeping stressed cells alive — tumor cells as readily as healthy ones. Blood levels track with family longevity and slower loss of thinking ability, though one study links higher levels to worse survival in the oldest. No trial has given it to a person; supply is unregulated. (Full Review)

Protocol

Human protocol
None validated
No approved product, dose, route, frequency or duration in humans.
Route
Injection
No oral bioavailability. Animal routes: intraperitoneal, intravenous, subcutaneous.
Preclinical reference
4 mg/kg twice weekly
S14G-humanin, 18-month-old mice, 14 months. Allometric human conversion is not validated.
Time to effect
In humans
Unknown
No perceptible acute effect is reported, so there is no early feedback signal.
Body composition
10 months
In aged mice, body composition was measured at 10 months of treatment.
Endurance exercise
30 min – 3 h
Endurance exercise acutely raises circulating humanin, at 30 min and 3 h.

Benefits

Contraindications
  • Active or recent (< 5 y) malignancy, premalignant lesion, hereditary cancer syndrome (BRCA1/2, Lynch)
  • Current or imminent curative-intent chemotherapy or radiotherapy (cyclophosphamide, cisplatin)
  • Pregnancy, lactation, or attempting conception
  • Children and adolescents before growth completion
  • Chronic kidney disease, eGFR < 30 mL/min/1.73 m²
  • Active proliferative disorder, e.g. untreated proliferative diabetic retinopathy
Key Interactions
  • Growth hormone and secretagogues (somatropin, tesamorelin, CJC-1295, ipamorelin, sermorelin)
  • Insulin and secretagogues (glargine, glipizide, glyburide, repaglinide)
  • Other glucose-lowering agents (metformin, semaglutide, tirzepatide, empagliflozin)
  • Glucose-lowering supplements (berberine, alpha-lipoic acid, cinnamon, Gymnema sylvestre)
  • Other mitochondrial peptides (MOTS-c, SS-31, humanin-like peptide 2)
  • Senolytics (dasatinib plus quercetin, fisetin)
  • Low-dose aspirin

Risk & Side Effects

  • High: Complete absence of human safety data; unregulated supply chain and product integrity
  • Medium: Tumor promotion and chemotherapy interference; growth axis and reproductive suppression
  • Low: Senescent cell inflammatory amplification; IGFBP-3 carrier perturbation; injection-related reactions
  • Speculative: Immunogenicity; chronic gp130/STAT3 activation; suppressed beneficial apoptosis and mitophagy

Monitoring

Marker Target Why
Fasting insulin 2–5 µIU/mL Primary target of the metabolic benefit
HOMA-IR < 1.0 Composite readout of insulin resistance
Fasting glucose 75–86 mg/dL Intended effect and hypoglycemia risk
HbA1c 4.8–5.4% Glycemic control; inversely tracks humanin
IGF-1 Age/sex mid-range Growth-axis suppression; avoid lowest quartile
IGFBP-3 Age-adjusted; ratio to IGF-1 Bound directly by humanin; most specific marker
hs-CRP < 0.5 mg/L Detects the senescence-amplification risk
Interleukin-6 < 2 pg/mL Rises with humanin in senescent cells
Triglycerides < 80 mg/dL Inversely correlated with humanin in human data
ApoB < 80 mg/dL (< 60 at high risk) Cardiovascular risk tracking
eGFR and creatinine eGFR > 90 mL/min/1.73 m² Renal function governs peptide clearance
ALT and AST < 25 U/L men, < 20 U/L women General safety screen for an unstudied compound
Complete blood count Reference range, lymphocytes Lymphocytes protected from cytotoxic injury

Cadence: Baseline within 4 weeks before use. Fasting glucose daily and a metabolic panel at 2 weeks; full panel at 12 weeks; then every 6 months. Age-appropriate cancer screening continues normally.

Qualitative Assessment

  • Cognitive clarity and working memory: strongest preclinical rationale; repeatable timed task.
  • Sleep quality and duration: a confounder, not an expected effect.
  • Energy and exercise capacity: perceived exertion at a fixed submaximal workload.
  • Body composition by feel and fit of clothing: detectable before it is measurable.
  • Recovery from training sessions: relevant to the muscle and glucocorticoid findings.
  • Injection site condition: persistent induration, erythema or nodules signal a product problem.