Audit: QRS - L-Cysteine for Health & Longevity

Audit conducted on 22/09/2026 18:03 using AI4L / Opus 5

Iterations

Summary

Items Count
Total 94
Passed 85
Failed 0
N/A 9
Pass Rate 100.00%
  • Total = Passed + Failed + N/A
  • Pass Rate = Passed / (Passed + Failed) × 100
  • N/A items are excluded from the pass rate calculation

1. General Rules

# Description Result Comments
1.1 Every claim, magnitude, label, recommendation, and statement in the QRS is literally supported by content in the source ER. 🟢 Spot-checked every populated span against the ER: protocol doses (3 mg lozenge, 700 mg L-cystine + 280 mg L-theanine, 1,200 mg), the eight biomarker targets, the seven contraindications and eight interactions all trace to literal ER text.
1.2 Where the ER uses cautious phrasing (“not formally studied”, “None documented in human trials to date”, “theoretical concern”, “data are limited”), the QRS uses the same phrasing. 🟢 Hedges are carried over: “No established target” for the glutathione ratio (ER line 454) and “the only regimen with abstinence data” (ER line 363).
1.3 The QRS never strengthens an ER claim (e.g., “not formally studied” → “not required”) or softens one (e.g., “do not use during pregnancy” → “use with caution during pregnancy”). 🟢 No strengthening or softening detected; pregnancy/lactation remains an avoid-population, and contested findings stay in the tiers the ER assigned them.
1.4 The QRS does not relabel an ER fact under a different decision category. A “Benefit-Modifying Factor” from ER section is not surfaced as a “Caution”; a “Risk-Modifying Factor” is not surfaced as a “Side Effect”; etc. 🟢 Contraindications come only from the ER’s “Populations who should avoid L-Cysteine” list; key interactions only from the ER interaction bullets; no modifying factor is repurposed as a gate.
1.5 PubMed IDs, study citations, expert names, clinical trial identifiers (NCT*), and brand names appear in the QRS only if they appear in the source ER for the same fact. 🟢 No PMIDs, author names, NCT identifiers or brand names appear anywhere in the QRS.
1.6 The QRS does not introduce new attributions. 🟢 No attribution of any kind is introduced.

2. Focus, Tone & Audience

# Description Result Comments
2.1 The QRS follows the tone of the ER, which is determined by the ER’s own language, phrasing, and framing. 🟢 Matches the ER’s measured, two-sided framing of a molecule with competing readings.
2.2 The tone of the QRS is simultaneously expert, accessible, objective, and data-driven, but also empowering and encouraging 🟢 Expert and data-driven while remaining accessible; the tiered benefit/risk structure keeps the sheet actionable.
2.3 The QRS reads as a trusted, knowledgeable guide rather than a prescriptive doctor 🟢 Descriptive throughout; no prescriptive instruction to a patient.
2.4 The QRS avoids language that implies medical or clinical advice 🟢 No imperative clinical direction; regimens are described (“Taken with every cigarette or drink”) rather than prescribed.
2.5 The QRS “presents information” instead of “providing guidance”, “recommending”, or “advising” 🟢 Information is presented; no “recommend”, “advise” or “should” appears in the QRS body.
2.6 The QRS never addresses “the reader” directly — it presents evidence, not guidance 🟢 No second-person pronoun appears anywhere in the sheet.
2.7 The QRS is written in plain language, avoiding unnecessary medical jargon 🟢 Technical terms are unavoidable domain vocabulary (cystinuria, HOMA-IR); phrasing is otherwise plain.
2.8 Information is presented in a concise and very compact manner 🟢 Every cell is a condensed fact; ER bullet explanations, em-dash clauses and the ER table’s Context/Notes column are all dropped.
2.9 It DOES NOT address the reader directly 🟢 Confirmed — no direct address.
2.10 The target audience is health- and longevity-oriented adults who are risk-aware, proactive, and actively seeking to optimize health or apply the intervention under review. 🟢 Content assumes a proactive optimizer: baseline panels, genotype gates and event-driven dosing.
2.11 The target audience is willing to employ lifestyle and behavioral changes as well as follow protocols that may be inconvenient, costly, or require effort. 🟢 Protocols presented (slow-release lozenges with every cigarette, 24-hour urine collection, split daily dosing) demand real effort.
2.12 The document is NOT written for the general population, who are unwilling to employ lifestyle and behavioral changes or follow protocols that may be inconvenient, costly, or require effort. 🟢 Not pitched at the general population; the framing assumes willingness to test and monitor.
2.13 Framing, takeaways, and risk/benefit weighting throughout the document reflect this audience, including where an intervention’s signal for the average person differs from its signal for this audience. 🟢 The adiposity/diabetes signal is surfaced as a Medium risk alongside monitoring markers, which is the weighting this audience needs.
2.14 The document’s own voice frames usage in longevity terms, not “anti-aging” (e.g., “anti-aging clinics”, “anti-aging community”, “anti-aging medicine”). Proper names that contain “anti-aging” (e.g., “American Academy of Anti-Aging Medicine”) are quoted verbatim. 🟢 The term “anti-aging” does not appear; the header uses “Health & Longevity”.
2.15 The document’s own voice uses formal clinical and scientific terminology, not colloquial or consumer-grade language (e.g., “oral medication” not “pill(s)”; “oral” / “administered orally” not “taken by mouth” / “given by mouth”; “injection” not “shot”; “adverse event” not “bad reaction”). This holds on EVERY surface, including the QRS lede — the stricter plain-language bar (see 2.7) does not license lay phrasing for route of administration. Direct quotes from sources are exempt. 🟢 Register stays clinical; no “pill”, “shot” or “taken by mouth”. “Lozenge”, “hangover” and “giving up smoking” are the ER’s own terms.

3. Template Integrity

# Description Result Comments
3.1 The following labels and headings on the QRS are fixed and not modified:
• Card and section headings: “Protocol”, “Time to effect”, “Benefits”, “Risk & Side Effects”, “Monitoring”, “Qualitative Assessment”
• Gate headings: “Contraindications”, “Key Interactions”
• Tier labels: “High”, “Medium”, “Low”, “Speculative”
• Table column headers in Monitoring: “Marker”, “Target”, “Why”
🟢 All fixed headings verified verbatim against the template: Protocol, Time to effect, Benefits, Risk & Side Effects, Monitoring, Qualitative Assessment, Contraindications, Key Interactions, the four tier labels and Marker/Target/Why.
3.2 All “<span data-qrs-var=”NAME”>…</span>” from the [qrs_template] are present in the the QRS. 🟢 All 34 fixed template variables are present, plus 8 marker rows (24 spans) and 6 qualitative spans — 64 in total, with none missing.
3.3 Spans that are not addressed in a checklist item are left unchanged 🟢 A diff against the template shows the stylesheet, the three website="..." spans, the footer disclaimer and all structural markup are byte-identical.

4. Formatting

# Description Result Comments
4.1 When the source ER section is empty, the QRS uses the ER’s own empty-state phrasing verbatim. Typical phrasings are “None documented in human trials to date” and “Not formally studied” N/A N/A — no section of the ER is empty; the High-risk tier carries explanatory prose, and its QRS span is handled under 13.5.
4.2 Where the ER presents a bulleted item as “Label: content”, the QRS uses the ER’s bold label verbatim as the cell or row label. 🟢 Protocol labels reuse the ER bold labels verbatim: “Acetaldehyde-binding lozenge”, “Immune regimen”, “Hangover regimen” (ER lines 363, 367, 369).
4.3 Labels are not paraphrased, abbreviated, or invented. 🟢 Biomarker names and protocol labels are taken verbatim; the three time-to-effect labels are drawn directly from the ER’s own wording in the Time to effect bullet.
4.4 The QRS DOES NOT use emoji indicators (no 🟩, 🟥, 🟨, etc.). Color and emphasis are conveyed through CSS and bold labels. 🟢 No emoji characters occur in the file; tiers are conveyed by bold labels and CSS only.
4.5 The QRS is designed to render on one A4 page. Any section that has more content in the ER than fits the per-section budget is condensed by the LLM, not extended onto a second page. 🟢 Content is condensed rather than transcribed — ER mechanism sentences, magnitudes, citations and the monitoring table’s Context/Notes column are all omitted to hold the one-page budget.

5. Metadata

# Description Result Comments
5.1 The metadata is placed inside a single HTML comment that is the first element after “<!doctype html>” and before any other comment, head, or body content. 🟢 The metadata comment opens on line 2, immediately after <!doctype html> on line 1, before any other markup.
5.2 Inside that HTML comment the YAML block is delimited by a line “—” opening and a line “—” closing. Text before the opening “—” is permitted but is not parsed as YAML. 🟢 YAML opens with --- on line 3 and closes with --- on line 13, inside the comment.
5.3 The metadata is not visible in any rendered view of the QRS and is not surfaced by any other element on the sheet. 🟢 The block is an HTML comment and is not echoed by any visible element.
5.4 All frontmatter values are trimmed: no leading or trailing whitespace, no surrounding quotes unless the value contains a colon, bracket, or leading special character that requires YAML quoting. 🟢 All values are trimmed; only duration: "00:02" is quoted, which is required because the value contains a colon.
5.5 The filename of the source ER is stated as “er_filename: [er_filename]” 🟢 Line 4: er_filename: l_cysteine_2026-0828-0556_Opus_ER.md.
5.6 Version of the QRS.md file used to create the document is stated as “qrs_prompt_version: [Version of QRS.md]” 🟢 Line 5: qrs_prompt_version: 26.9.22, matching the version badge of QRS.md.
5.7 Creation date and time of the document is stated as “qrs_creation_date: [YYYY-MMDD-HHMM]” (e.g., 2026-0501-1430) 🟢 Line 6: qrs_creation_date: 2026-0922-1757.
5.8 The nickname of the AI used to create the document is stated as “qrs_creator_ai_nickname: [qrs_creator_ai_nickname]” 🟢 Line 7: qrs_creator_ai_nickname: Opus.
5.9 The nickname of the AI is just a single word model name without version, etc. (e.g., Opus, Sonnet, Grok, Gemini, ChatGPT) 🟢 “Opus” is a single word with no version or qualifier.
5.10 The full name of the AI used to create the document is stated as “qrs_creator_ai_fullname: [qrs_creator_ai_fullname]” 🟢 Line 8: qrs_creator_ai_fullname: Opus 5.
5.11 The full name of the AI consists of the [qrs_creator_ai_nickname] and the model version number and no additional qualifier (e.g., Opus 4.6, Sonnet 3.2, Grok 4.5, Gemini 3.1, ChatGPT 5.4) 🟢 “Opus 5” is nickname plus version number, no qualifier.
5.12 The filename of the document is stated as “qrs_filename: [filename of this document]” 🟢 Line 9: qrs_filename: l_cysteine_2026-0828-0556_Opus_QRS.html, matching the file on disk.
5.13 All frontmatter values are trimmed: no leading or trailing whitespace, no surrounding quotes unless the value contains a colon, bracket, or leading special character that requires YAML quoting. 🟢 Re-verified: no stray whitespace and no unnecessary quoting.

6. Page Title & Header

# Description Result Comments
6.1 [page_title] is set to the [canonical_topic] of the ER frontmatter followed by “ - Quick Reference Sheet” (e.g., “Intervention - Quick Reference Sheet”). The [canonical_topic] is HTML-entity-encoded as needed (e.g., &amp; for &) 🟢 Line 22: L-Cysteine for Health &amp; Longevity - Quick Reference Sheet, with the ampersand entity-encoded.
6.2 [header_topic] is set to the [canonical_topic] of the ER frontmatter, with HTML entities encoded as needed (e.g., &amp; for &) 🟢 Line 417: L-Cysteine for Health &amp; Longevity, matching canonical_topic in the ER frontmatter.
6.3 [header_subline_date] is set to [qrs_creation_date reformatted as MM/DD/YYYY] 🟢 Line 421: 09/22/2026, the MM/DD/YYYY form of 2026-0922-1757.
6.4 [header_subline_model] is set to [qrs_creator_ai_fullname] 🟢 Line 425: Opus 5.
6.5 No additional header content appears: no badge, version stamp, AKA / alternate names line, source-AI attribution, audit date, or QRS variant marker. 🟢 The subline carries only the creation date, the AI4L link and the model name; the ER’s alternate-names line is not reproduced.

7. At-A-Glance Section

# Description Result Comments
7.1 [at_a_glance] opens by saying what the intervention is — its kind (e.g., dietary supplement, prescription medication, peptide, plant, procedure, practice) — and what it is used for, in plain language, before any verdict on the evidence 🟢 Opens “L-Cysteine is a sulfur-containing amino acid sold as a supplement to build the body’s main internal antioxidant and to neutralize the toxic by-product of alcohol and tobacco smoke” — kind and purpose before any verdict.
7.2 [at_a_glance] is dense, execution-oriented summary of the ER Conclusion section 🟢 Condenses the ER Conclusion’s three moves: the two roles, the better-supported chemical one, and the contested antioxidant one with the adiposity counter-signal.
7.3 [at_a_glance] is no longer than 70 words 🟢 68 words, verified by script.
7.4 Every fact in [at_a_glance] is supported by a distinct passage in the ER. 🟢 Each clause maps to ER lines 492–494 of the Conclusion.
7.5 It DOES NOT use acronyms or technical classifications that require specialist knowledge, uses plain-language terms instead 🟢 No acronyms; “main internal antioxidant” stands in for glutathione and “toxic by-product” for acetaldehyde.
7.6 It DOES NOT cite specific trials (names, years, sample sizes, p-values) 🟢 No trial names, years, sample sizes or p-values.
7.7 It DOES NOT cite effect sizes, relative risks, or statistical results 🟢 No odds ratios, confidence intervals or effect sizes.

8. Contraindications

# Description Result Comments
8.1 The section is derived from the ER Key Interactions & Contraindications section 🟢 Drawn from the ER’s “Populations who should avoid L-Cysteine” list inside Key Interactions & Contraindications (ER lines 331–339).
8.2 [stop_items] represent the Contraindications from the ER 🟢 All seven ER avoid-populations are present, plus supervised disulfiram, which the ER labels an absolute contraindication.
8.3 Individual [stop_items] are formatted as <li></li> 🟢 Seven <li> elements inside the stop_items span, verified by script.
8.4 Items are as concise as possible. No trailing explanations, no elaborations, no mechanistic rationale, no attributions, no citations, no study details. No content after an em-dash, en-dash, or hyphen-dash (e.g., “— dose reduction required”, “— reduced efficacy”) — these trailing clauses are stripped. Just the key fact. 🟢 Trailing explanations are stripped — the ER’s em-dash gloss on the sulfur-clearance disorders and “a measure of kidney filtering capacity” are both removed.
8.5 Parenthetical qualifiers from the ER bullet — time windows, severity classes, threshold values, clinical staging — ARE preserved as part of the item, kept as concise as possible (shortened or trimmed where needed to fit the one-page budget, but never dropped entirely). 🟢 Qualifiers survive: “(type A, B or AB)”, “above 250 mg/g creatinine”, “stage 4 or 5”, “below 30 mL/min/1.73 m²”, “(Child-Pugh Class C)”.
8.6 When the ER uses ranking notation inside parens (e.g., “>” for severity ordering) that depends on an explanatory phrase to interpret, normalize the items to a plain comma-separated list rather than carrying through the bare symbol. N/A N/A — the ER’s contraindication bullets use no ranking notation inside parentheses.
8.7 The section is left empty ONLY IF the ER’s Key Interactions & Contraindications section identifies no population, condition, or scenario for which the intervention should be avoided, deferred, or used only under specialist supervision. 🟢 The section is populated, and the ER does name populations that must avoid the intervention, so the empty case does not arise.
8.8 If the section is left empty, an HTML comment inside the [stop_items] span records the ER basis for the absence, naming or quoting the ER text relied on. E.g. <!– empty: ER names no population that should avoid the intervention –> N/A N/A — the section is not empty; seven contraindications are listed.

9. Key Interactions

# Description Result Comments
9.1 The section is derived from the ER Key Interactions & Contraindications section 🟢 Drawn from the ER’s interaction bullets (ER lines 313–329).
9.2 [caution_items] represent the Key Interactions from the ER, excluding any that are already listed as Contraindications 🟢 Eight interactions carried over; disulfiram is correctly excluded because it sits in Contraindications, and the oncology-protocol clause of the final bullet is likewise excluded as a contraindication.
9.3 Individual [caution_items] are formatted as <li></li> 🟢 Eight <li> elements inside the caution_items span, verified by script.
9.4 Items are as concise as possible. No trailing explanations, no elaborations, no mechanistic rationale, no attributions, no citations, no study details. No content after an em-dash, en-dash, or hyphen-dash (e.g., “— dose reduction required”, “— reduced efficacy”) — these trailing clauses are stripped. Just the key fact. 🟢 Every ER em-dash rationale (“Caution — thiols regenerate the nitrate response…”) is stripped; only the agent class remains.
9.5 Parenthetical qualifiers from the ER bullet — example drug lists, time windows, severity classes, threshold values, clinical staging — ARE preserved as part of the item, kept as concise as possible (shortened or trimmed where needed to fit the one-page budget, but never dropped entirely). 🟢 Named example drugs are retained in full for nitrates, proton pump inhibitors, cystine-stone medications, acid reducers and the additive sulfur-amino-acid list.
9.6 When the ER uses ranking notation inside parens (e.g., “>” for severity ordering) that depends on an explanatory phrase to interpret, normalize the items to a plain comma-separated list rather than carrying through the bare symbol. N/A N/A — the ER’s interaction bullets use no ranking notation inside parentheses.
9.7 The section is left empty ONLY IF the ER’s Key Interactions & Contraindications section identifies no interaction, additive effect, or exposure that changes how the intervention is used. 🟢 The section is populated, and the ER does name interactions that change how the intervention is used.
9.8 If the section is left empty, an HTML comment inside the [caution_items] span records the ER basis for the absence, naming or quoting the ER text relied on. E.g. <!– empty: ER names no interaction that changes how the intervention is used –> N/A N/A — the section is not empty; eight key interactions are listed.

10. Protocol

# Description Result Comments
10.1 The section is derived from the ER Protocol section 🟢 Derived from the ER Therapeutic Protocol section (ER lines 361–387).
10.2 The three sets of [action] items cover the three most important actionable implementation aspects from the ER Protocol section 🟢 The three chosen regimens are the lozenge (the only one with abstinence data), the immune regimen (the sole Medium-tier benefit) and the hangover regimen; the 200 mg gastric capsule is the weaker Low-tier variant of the same acetaldehyde mechanism.
10.3 If less that three distinct actionable implementation aspects are mentioned in the ER the unused sets are left empty and made invisible, not filled with placeholder text or empty-state phrasing. N/A N/A — the ER supplies four distinct protocol regimens, so all three action sets are used.
10.4 All used [action_#label], [action#value], [action#_sub] items are filled with meaningful content derived from the ER Protocol section. 🟢 All nine action spans carry ER-derived content, including the dose figures and the “split after breakfast and after dinner” schedule.

11. Time to Effect

# Description Result Comments
11.1 The three sets of [time] items cover the three most important time-to-effect aspects from the ER 🟢 Covers all three aspects the ER’s Time to effect bullet names: immediate acetaldehyde binding, 2 weeks for biochemistry, 12 to 16 weeks for function (ER line 422).
11.2 The sets are picked and ordered by the magnitude of the related benefit 🟢 Acetaldehyde binding sits first, tied to the High-tier smoking-cessation benefit; the two glutathione aspects follow in the ER’s own biochemistry-then-function order.
11.3 If less that three distinct time-to-effect aspects are mentioned in the ER the unused sets are left empty and made invisible, not filled with placeholder text or empty-state phrasing. N/A N/A — the ER supplies three distinct time-to-effect aspects, so all three time sets are used.
11.4 All used [time_#label], [time#value], [time#_sub] items are filled with meaningful content derived from the ER. 🟢 Labels, values and subs are all ER-derived, including “Lasts about three hours per dose” and “Gait speed and strength in older adults”.
11.5 If the ER does not provide any information on time to effect, the section is removed completely from the Protocol Panel N/A N/A — the ER’s Practical Considerations section provides a Time to effect bullet, so the row is retained.

12. Benefits

# Description Result Comments
12.1 The section is derived from the ER Expected Benefits section 🟢 Derived from the ER Expected Benefits section (ER lines 155–221).
12.2 Key variables are [benefits_high], [benefits_medium], [benefits_low], [benefits_speculative] 🟢 All four benefit variables are present and populated.
12.3 Items are as concise as possible. No explanations, no elaborations, no effect sizes, no qualifiers, no attributions, no citations, no study details, no mechanistic explanations, etc. Just the key fact. 🟢 Each entry is the ER’s benefit heading reduced to a clause; no magnitudes, mechanisms or trial details carry over.
12.4 Parenthetical content — including effect sizes, sample notes, mechanistic hints, and example studies — is stripped, NOT preserved. 🟢 The ER’s headings contain no parentheticals, and none of the Magnitude parentheses are reproduced.
12.5 If no items of a specific sub-section (high, medium, low, speculative) are present the respective <SPAN> is set to “display=none”, not filled with “None documented in human trials to date” or similar empty-state phrasing. N/A N/A — all four benefit tiers are populated in the ER, so no benefit span needed hiding.

13. Risks

# Description Result Comments
13.1 The section is derived from the ER Potential Risks & Side Effects section 🟢 Derived from the ER Potential Risks & Side Effects section (ER lines 243–293).
13.2 Key variables are [risks_high], [risks_medium], [risks_low], [risks_speculative] 🟢 All four risk variables are present; risks_high is retained as an empty hidden span.
13.3 Items are as concise as possible. No explanations, no elaborations, no effect sizes, no qualifiers, no attributions, no citations, no study details, no mechanistic explanations, etc. Just the key fact. 🟢 Each entry is the ER’s risk heading reduced to a clause; the “⚠️ Conflicted” marker and all magnitudes are dropped, as item 13.3 requires.
13.4 Parenthetical content — including frequencies, severity grades, sample notes, mechanistic hints, and example studies — is stripped, NOT preserved. 🟢 No parenthetical content from the ER risk section is carried over.
13.5 If no items of a specific sub-section (high, medium, low, speculative) are present the respective <SPAN> is set to “display=none”, not filled with “None documented in human trials to date” or similar empty-state phrasing. 🟢 The ER states “No risk reaches High”, and line 614 correctly sets risks_high to style="display: none" with no empty-state text.

14. Monitoring

# Description Result Comments
14.1 The section is derived from the ER Monitoring section 🟢 Derived from the ER Monitoring Protocol & Defining Success table (ER lines 450–459).
14.2 All measurable/quantifiable biomarkers from the Monitoring section are listed 🟢 All eight ER biomarkers are present in the same order, with targets transcribed exactly (200–260 µmol/L, below 8 µmol/L, insulin below 5 µIU/mL, HOMA-IR below 1.5, 4.8–5.4%, 94/80 cm, 24–28 mmol/L, below 100 mg per day).
14.3 [monitoring_cadence] is populated with the monitoring cadence/frequency derived from the ER Monitoring section. It is not left with placeholder text or empty. 🟢 Populated with the ER’s cadence from line 448: baseline before any daily regimen, metabolic markers at 12 weeks then every 6 months, waist circumference every 3 months.

15. Qualitative Assessment

# Description Result Comments
15.1 The section is derived from the ER Monitoring section 🟢 Derived from the qualitative-marker list in the ER Monitoring Protocol & Defining Success section (ER lines 461–468).
15.2 All subjective/qualitative biomarkers from the Monitoring section are listed 🟢 All six qualitative markers are present and transcribed verbatim.

Issues 22/09/2026 18:03

Pass rate 100.00%. No issues found.