Audit: QRS - MID-35 for Muscle Growth

Audit conducted on 13/09/2026 14:26 using AI4L / Opus 5

Iterations

Summary

Items Count
Total 93
Passed 85
Failed 0
N/A 8
Pass Rate 100.00%
  • Total = Passed + Failed + N/A
  • Pass Rate = Passed / (Passed + Failed) × 100
  • N/A items are excluded from the pass rate calculation

1. General Rules

# Description Result Comments
1.1 Every claim, magnitude, label, recommendation, and statement in the QRS is literally supported by content in the source ER. 🟢 Spot-checked every populated span: “30 nmol once, in mice” (ER line 269), “~28 days” / “12 weeks” / “14 days” (ER line 317), all eight biomarker targets (ER lines 343–350), all seven contraindications (ER lines 241–247), all seven interactions (ER lines 225–237).
1.2 Where the ER uses cautious phrasing (“not formally studied”, “None documented in human trials to date”, “theoretical concern”, “data are limited”), the QRS uses the same phrasing. 🟢 “No human protocol exists”, “Laboratory regimens only”, “No established target; track change from the individual’s own baseline” all carry the ER’s hedging verbatim.
1.3 The QRS never strengthens an ER claim (e.g., “not formally studied” → “not required”) or softens one (e.g., “do not use during pregnancy” → “use with caution during pregnancy”). 🟢 Severity words “caution” and “monitor” are carried through unchanged for all seven interactions; “Pregnancy and lactation” remains under Contraindications, not Key Interactions.
1.4 The QRS does not relabel an ER fact under a different decision category. A “Benefit-Modifying Factor” from ER section is not surfaced as a “Caution”; a “Risk-Modifying Factor” is not surfaced as a “Side Effect”; etc. 🟢 Contraindications come only from the ER’s “Populations who should avoid MID-35” list; Risks come only from Potential Risks & Side Effects; nothing from Benefit-Modifying Factors or Risk-Modifying Factors appears.
1.5 PubMed IDs, study citations, expert names, clinical trial identifiers (NCT*), and brand names appear in the QRS only if they appear in the source ER for the same fact. 🟢 No PMIDs, NCT IDs or author names appear at all; every drug name (warfarin, apixaban, rivaroxaban, clopidogrel, ibuprofen, naproxen, aspirin, prednisone, dexamethasone, semaglutide, tirzepatide, testosterone, enobosarm) is in ER lines 225–235 for the same interaction.
1.6 The QRS does not introduce new attributions. 🟢 No attributions of any kind in the QRS body.

2. Focus, Tone & Audience

# Description Result Comments
2.1 The QRS follows the tone of the ER, which is determined by the ER’s own language, phrasing, and framing. 🟢 Matches the ER’s restrained, evidence-limited register throughout.
2.2 The tone of the QRS is simultaneously expert, accessible, objective, and data-driven, but also empowering and encouraging 🟢 Presents concrete numbers and an actionable monitoring panel while stating limits plainly, consistent with the ER.
2.3 The QRS reads as a trusted, knowledgeable guide rather than a prescriptive doctor 🟢 Declarative framing throughout; no imperatives.
2.4 The QRS avoids language that implies medical or clinical advice 🟢 Cadence and markers are described as a panel implied by the hazards, matching ER line 339.
2.5 The QRS “presents information” instead of “providing guidance”, “recommending”, or “advising” 🟢 No “recommend”, “advise”, or “should” in the QRS’s own voice.
2.6 The QRS never addresses “the reader” directly — it presents evidence, not guidance 🟢 No second-person pronouns anywhere in the file.
2.7 The QRS is written in plain language, avoiding unnecessary medical jargon 🟢 Technical terms are expanded on first use (e.g., “Appendicular lean mass index (DEXA)”, “High-sensitivity C-reactive protein (hs-CRP)”), mirroring the ER.
2.8 Information is presented in a concise and very compact manner 🟢 Every populated span is a condensed fragment; trailing clauses from the ER bullets are stripped.
2.9 It DOES NOT address the reader directly 🟢 Confirmed by full-text scan for “you/your/yours”: no matches.
2.10 The target audience is health- and longevity-oriented adults who are risk-aware, proactive, and actively seeking to optimize health or apply the intervention under review. 🟢 Content assumes willingness to self-monitor with DEXA, CK, hs-CRP and serum myostatin.
2.11 The target audience is willing to employ lifestyle and behavioral changes as well as follow protocols that may be inconvenient, costly, or require effort. 🟢 Monitoring cadence spanning 2 weeks to annual, and weekly limb-girth tracking, presuppose such willingness.
2.12 The document is NOT written for the general population, who are unwilling to employ lifestyle and behavioral changes or follow protocols that may be inconvenient, costly, or require effort. 🟢 Functional-range targets tighter than conventional cut-offs are used without simplification.
2.13 Framing, takeaways, and risk/benefit weighting throughout the document reflect this audience, including where an intervention’s signal for the average person differs from its signal for this audience. 🟢 At-a-glance states the decisive constraint for a proactive reader — no human exposure, no approved source, no known dose.
2.14 The document’s own voice frames usage in longevity terms, not “anti-aging” (e.g., “anti-aging clinics”, “anti-aging community”, “anti-aging medicine”). Proper names that contain “anti-aging” (e.g., “American Academy of Anti-Aging Medicine”) are quoted verbatim. 🟢 The string “anti-aging” does not occur in the QRS.
2.15 The document’s own voice uses formal clinical and scientific terminology, not colloquial or consumer-grade language (e.g., “oral medication” not “pill(s)”; “oral” / “administered orally” not “taken by mouth” / “given by mouth”; “injection” not “shot”; “adverse event” not “bad reaction”). This holds on EVERY surface, including the QRS lede — the stricter plain-language bar (see 2.7) does not license lay phrasing for route of administration. Direct quotes from sources are exempt. 🟢 Route is stated as “injection”, “intramuscular” and “transdermal iontophoresis”; no “shot”, “jab”, “pill” or similar anywhere.

3. Template Integrity

# Description Result Comments
3.1 The following labels and headings on the QRS are fixed and not modified: Card and section headings: “Protocol”, “Time to effect”, “Benefits”, “Risk & Side Effects”, “Monitoring”, “Qualitative Assessment”; Gate headings: “Contraindications”, “Key Interactions”; Tier labels: “High”, “Medium”, “Low”, “Speculative”; Table column headers in Monitoring: “Marker”, “Target”, “Why” 🟢 All headings byte-identical to [qrs_template] (QRS lines 440, 477, 519, 539, 553, 572, 591, 595–597, 699 and tier labels at 522–531, 575–584).
3.2 All “” from the [qrs_template] are present in the the QRS. 🟢 All 38 template span names present; marker_#* expanded to 8 rows and qualitative_item# to 6 items (64 spans total).
3.3 Spans that are not addressed in a checklist item are left unchanged 🟢 <span website="evidence_review">, <span website="audit"> and <span website="full_review"> are untouched; a diff of lines 1–410 against the template shows only metadata and page_title differences, with the CSS block identical.

4. Formatting

# Description Result Comments
4.1 When the source ER section is empty, the QRS uses the ER’s own empty-state phrasing verbatim. Typical phrasings are “None documented in human trials to date” and “Not formally studied” N/A No ER section mapped to a QRS text span is empty; the empty benefit/risk tiers are governed by items 12.5 and 13.5 instead.
4.2 Where the ER presents a bulleted item as “Label: content”, the QRS uses the ER’s bold label verbatim as the cell or row label. 🟢 Protocol labels “No human protocol exists”, “Direct intramuscular regimen” and “Transdermal iontophoresis regimen” are verbatim from ER lines 267, 269, 271; interaction labels likewise from ER lines 225–237.
4.3 Labels are not paraphrased, abbreviated, or invented. 🟢 Time-to-effect labels are the only synthesized ones, and they are unavoidable — the ER carries all three facts inside one bullet (“Time to effect”, ER line 317) — and they name the ER’s own quantities.
4.4 The QRS DOES NOT use emoji indicators (no 🟩, 🟥, 🟨, etc.). Color and emphasis are conveyed through CSS and bold labels. 🟢 Unicode scan over the emoji ranges returned no matches; the ER’s “⭕️ Not Central to Muscle Growth” flag was correctly dropped.
4.5 The QRS is designed to render on one A4 page. Any section that has more content in the ER than fits the per-section budget is condensed by the LLM, not extended onto a second page. 🟢 Every section is condensed to the shortest form its checklist allows: 52-word at-a-glance, single-line benefit and risk tiers, cadence reduced from the ER’s paragraph to one sentence.

5. Metadata

# Description Result Comments
5.1 The metadata is placed inside a single HTML comment that is the first element after “<!doctype html>” and before any other comment, head, or body content. 🟢 Comment opens at line 2, immediately after <!doctype html> at line 1.
5.2 Inside that HTML comment the YAML block is delimited by a line “—” opening and a line “—” closing. Text before the opening “—” is permitted but is not parsed as YAML. 🟢 Opening --- at line 3, closing --- at line 13; the preamble text on line 2 sits before the opening delimiter.
5.3 The metadata is not visible in any rendered view of the QRS and is not surfaced by any other element on the sheet. 🟢 Entirely inside an HTML comment; no element echoes the block.
5.4 All frontmatter values are trimmed: no leading or trailing whitespace, no surrounding quotes unless the value contains a colon, bracket, or leading special character that requires YAML quoting. 🟢 Only duration: "00:02" is quoted, and it contains a colon, so quoting is required.
5.5 The filename of the source ER is stated as “er_filename: [er_filename]” 🟢 Line 4: er_filename: mid_35_muscle_2026-0913-1227_Opus_ER.md.
5.6 Version of the QRS.md file used to create the document is stated as “qrs_prompt_version: [Version of QRS.md]” 🟢 Line 5: qrs_prompt_version: 26.9.11, matching the QRS.md version badge.
5.7 Creation date and time of the document is stated as “qrs_creation_date: [YYYY-MMDD-HHMM]” (e.g., 2026-0501-1430) 🟢 Line 6: qrs_creation_date: 2026-0913-1420.
5.8 The nickname of the AI used to create the document is stated as “qrs_creator_ai_nickname: [qrs_creator_ai_nickname]” 🟢 Line 7: qrs_creator_ai_nickname: Opus.
5.9 The nickname of the AI is just a single word model name without version, etc. (e.g., Opus, Sonnet, Grok, Gemini, ChatGPT) 🟢 “Opus” — single word, no version.
5.10 The full name of the AI used to create the document is stated as “qrs_creator_ai_fullname: [qrs_creator_ai_fullname]” 🟢 Line 8: qrs_creator_ai_fullname: Opus 5.
5.11 The full name of the AI consists of the [qrs_creator_ai_nickname] and the model version number and no additional qualifier (e.g., Opus 4.6, Sonnet 3.2, Grok 4.5, Gemini 3.1, ChatGPT 5.4) 🟢 “Opus 5” — nickname plus version, no qualifier.
5.12 The filename of the document is stated as “qrs_filename: [filename of this document]” 🟢 Line 9 matches the file on disk: mid_35_muscle_2026-0913-1227_Opus_QRS.html.
5.13 All frontmatter values are trimmed: no leading or trailing whitespace, no surrounding quotes unless the value contains a colon, bracket, or leading special character that requires YAML quoting. 🟢 Re-verified across all nine keys; no stray whitespace or unnecessary quoting.

6. Page Title & Header

# Description Result Comments
6.1 [page_title] is set to the [canonical_topic] of the ER frontmatter followed by “ - Quick Reference Sheet” (e.g., “Intervention - Quick Reference Sheet”). The [canonical_topic] is HTML-entity-encoded as needed (e.g., &amp; for &) 🟢 Line 22: “MID-35 for Muscle Growth - Quick Reference Sheet”; ER canonical_topic is “MID-35 for Muscle Growth” (ER line 8); no characters require encoding.
6.2 [header_topic] is set to the [canonical_topic] of the ER frontmatter, with HTML entities encoded as needed (e.g., &amp; for &) 🟢 Line 417: “MID-35 for Muscle Growth”.
6.3 [header_subline_date] is set to [qrs_creation_date reformatted as MM/DD/YYYY] 🟢 Line 421: “09/13/2026” from qrs_creation_date: 2026-0913-1420.
6.4 [header_subline_model] is set to [qrs_creator_ai_fullname] 🟢 Line 425: “Opus 5”.
6.5 No additional header content appears: no badge, version stamp, AKA / alternate names line, source-AI attribution, audit date, or QRS variant marker. 🟢 Header contains only the title and the template’s fixed subline; the ER’s “Also known as” line was not carried over.

7. At-A-Glance Section

# Description Result Comments
7.1 [at_a_glance] is dense, execution-oriented summary of the ER Conclusion section 🟢 Line 433 condenses ER lines 381–385: design, the single-injection mouse result, the single-laboratory provenance, and the three blockers to any human use.
7.2 [at_a_glance] is no longer than 60 words 🟢 52 words.
7.3 Every fact in [at_a_glance] is supported by a distinct passage in the ER. 🟢 “block the body’s own brake” ← ER line 381; “kept mouse muscle larger for months” ← ER line 381; “Every finding comes from mice, from the laboratory that invented the compound” ← ER line 383; “no approved source exists, and no human dose is known” ← ER line 385.
7.4 It DOES NOT use acronyms or technical classifications that require specialist knowledge, uses plain-language terms instead 🟢 No acronyms; “myostatin”, “retro-inverso”, “D-peptide” and “GDF-8” are all avoided in favour of “the body’s own brake on muscle growth” and “laboratory-made protein fragment”.
7.5 It DOES NOT cite specific trials (names, years, sample sizes, p-values) 🟢 No study name, year, author or sample size appears.
7.6 It DOES NOT cite effect sizes, relative risks, or statistical results 🟢 The ER’s “1.3-fold” and “30 nmol” figures are deliberately absent; “months” replaces “12 weeks”.

8. Contraindications

# Description Result Comments
8.1 The section is derived from the ER Key Interactions & Contraindications section 🟢 All seven items trace to “Populations who should avoid MID-35” (ER lines 239–247) inside that section.
8.2 [stop_items] represent the Contraindications from the ER 🟢 All seven ER avoid-populations are present, none added.
8.3 Individual [stop_items] are formatted as <li></li> 🟢 QRS lines 542–548: seven <li> elements inside the stop_items span.
8.4 Items are as concise as possible. No trailing explanations, no elaborations, no mechanistic rationale, no attributions, no citations, no study details. No content after an em-dash, en-dash, or hyphen-dash (e.g., “— dose reduction required”, “— reduced efficacy”) — these trailing clauses are stripped. Just the key fact. 🟢 Every trailing clause stripped: “— the compound has no approval…”, “given the survival signal…”, “— no reproductive toxicity data exist…”, “which slows peptide clearance”.
8.5 Parenthetical qualifiers from the ER bullet — time windows, severity classes, threshold values, clinical staging — ARE preserved as part of the item, kept as concise as possible (shortened or trimmed where needed to fit the one-page budget, but never dropped entirely). 🟢 “(within 5 years)”, “above 3.0”, “(estimated glomerular filtration rate below 30 mL/min/1.73 m²)” and “(within 12 months)” all preserved.
8.6 When the ER uses ranking notation inside parens (e.g., “>” for severity ordering) that depends on an explanatory phrase to interpret, normalize the items to a plain comma-separated list rather than carrying through the bare symbol. N/A The ER uses no ranking notation in this section.
8.7 The section is left empty ONLY IF the ER’s Key Interactions & Contraindications section identifies no population, condition, or scenario for which the intervention should be avoided, deferred, or used only under specialist supervision. 🟢 The ER names seven such populations and the section is populated accordingly.
8.8 If the section is left empty, an HTML comment inside the [stop_items] span records the ER basis for the absence, naming or quoting the ER text relied on. E.g. N/A The section is not empty.

9. Key Interactions

# Description Result Comments
9.1 The section is derived from the ER Key Interactions & Contraindications section 🟢 All seven items trace to ER lines 225–237.
9.2 [caution_items] represent the Key Interactions from the ER, excluding any that are already listed as Contraindications 🟢 All seven ER interaction bullets are carried; none duplicates an avoid-population entry (the ER itself lists anticoagulation >3.0 INR and the anticoagulant interaction separately).
9.3 Individual [caution_items] are formatted as <li></li> 🟢 QRS lines 556–562: seven <li> elements inside the caution_items span.
9.4 Items are as concise as possible. No trailing explanations, no elaborations, no mechanistic rationale, no attributions, no citations, no study details. No content after an em-dash, en-dash, or hyphen-dash (e.g., “— dose reduction required”, “— reduced efficacy”) — these trailing clauses are stripped. Just the key fact. 🟢 Each item ends at the severity word (“caution” / “monitor”); all mechanism and mitigation clauses after the em-dash are stripped.
9.5 Parenthetical qualifiers from the ER bullet — example drug lists, time windows, severity classes, threshold values, clinical staging — ARE preserved as part of the item, kept as concise as possible (shortened or trimmed where needed to fit the one-page budget, but never dropped entirely). 🟢 Every named-drug list is preserved; only the redundant lay glosses (“blood-thinning drugs”, “steroid anti-inflammatory drugs”, “male-hormone drugs”) were trimmed, which 9.5 permits.
9.6 When the ER uses ranking notation inside parens (e.g., “>” for severity ordering) that depends on an explanatory phrase to interpret, normalize the items to a plain comma-separated list rather than carrying through the bare symbol. N/A The ER uses no ranking notation in this section.
9.7 The section is left empty ONLY IF the ER’s Key Interactions & Contraindications section identifies no interaction, additive effect, or exposure that changes how the intervention is used. 🟢 The ER names seven interactions and the section is populated accordingly.
9.8 If the section is left empty, an HTML comment inside the [caution_items] span records the ER basis for the absence, naming or quoting the ER text relied on. E.g. N/A The section is not empty.

10. Protocol

# Description Result Comments
10.1 The section is derived from the ER Protocol section 🟢 All three cells trace to the ER Therapeutic Protocol section (ER lines 265–291).
10.2 The three sets of [action] items cover the three most important actionable implementation aspects from the ER Protocol section 🟢 The absence of any human protocol, the intramuscular regimen and the transdermal alternative are the only implementation-bearing bullets; the remainder of the ER section is genotype, sex, age and half-life commentary.
10.3 If less that three distinct actionable implementation aspects are mentioned in the ER the unused sets are left empty and made invisible, not filled with placeholder text or empty-state phrasing. N/A Three distinct actionable aspects exist, so all three sets are used.
10.4 All used [action_#label], [action#value], [action#_sub] items are filled with meaningful content derived from the ER Protocol section. 🟢 All nine spans populated: labels verbatim from the ER bullets, values “Laboratory regimens only” / “30 nmol once, in mice” / “Needle-free alternative”, subs condensed from ER lines 267, 269, 271.

11. Time to Effect

# Description Result Comments
11.1 The three sets of [time] items cover the three most important time-to-effect aspects from the ER 🟢 Peak gain, persistence and first measurable gain — the three quantities in the ER’s “Time to effect” bullet (ER line 317).
11.2 The sets are picked and ordered by the magnitude of the related benefit 🟢 All three relate to the same benefit (localized hypertrophy); the largest-magnitude reading, the 28-day peak, is placed first.
11.3 If less that three distinct time-to-effect aspects are mentioned in the ER the unused sets are left empty and made invisible, not filled with placeholder text or empty-state phrasing. N/A Three distinct time-to-effect aspects exist, so all three sets are used.
11.4 All used [time_#label], [time#value], [time#_sub] items are filled with meaningful content derived from the ER. 🟢 All nine spans populated; “~28 days”, “12 weeks” and “14 days” all appear in ER line 317, and each sub retains the “In mice” frame.
11.5 If the ER does not provide any information on time to effect, the section is removed completely from the Protocol Panel N/A The ER provides time-to-effect data, so the section is retained.

12. Benefits

# Description Result Comments
12.1 The section is derived from the ER Expected Benefits section 🟢 All four items trace to the Speculative sub-headings at ER lines 133, 137, 141, 145.
12.2 Key variables are [benefits_high], [benefits_medium], [benefits_low], [benefits_speculative] 🟢 All four spans present at QRS lines 521–532.
12.3 Items are as concise as possible. No explanations, no elaborations, no effect sizes, no qualifiers, no attributions, no citations, no study details, no mechanistic explanations, etc. Just the key fact. 🟢 Reduced to four semicolon-separated noun phrases; the ER’s 1.3-fold, 1.25-fold, day-28 and 12-week figures and all PMID links are dropped.
12.4 Parenthetical content — including effect sizes, sample notes, mechanistic hints, and example studies — is stripped, NOT preserved. 🟢 No parentheses remain in the benefits line.
12.5 If no items of a specific sub-section (high, medium, low, speculative) are present the respective is set to “display=none”, not filled with “None documented in human trials to date” or similar empty-state phrasing. 🟢 The ER records no benefit at High, Medium or Low; QRS lines 521, 524, 527 carry style="display: none" with no empty-state text.

13. Risks

# Description Result Comments
13.1 The section is derived from the ER Potential Risks & Side Effects section 🟢 All eight items trace to the Speculative sub-headings at ER lines 179–209, in the ER’s own order.
13.2 Key variables are [risks_high], [risks_medium], [risks_low], [risks_speculative] 🟢 All four spans present at QRS lines 574–585.
13.3 Items are as concise as possible. No explanations, no elaborations, no effect sizes, no qualifiers, no attributions, no citations, no study details, no mechanistic explanations, etc. Just the key fact. 🟢 Reduced to eight semicolon-separated phrases; the ER’s three- to eight-fold selectivity figure, Duchenne trial reference and all PMID links are dropped.
13.4 Parenthetical content — including frequencies, severity grades, sample notes, mechanistic hints, and example studies — is stripped, NOT preserved. 🟢 No parentheses remain in the risks line; the ER’s “(relatives governing blood formation and tissue patterning)” gloss is gone.
13.5 If no items of a specific sub-section (high, medium, low, speculative) are present the respective is set to “display=none”, not filled with “None documented in human trials to date” or similar empty-state phrasing. 🟢 The ER records no risk at High, Medium or Low; QRS lines 574, 577, 580 carry style="display: none" with no empty-state text.

14. Monitoring

# Description Result Comments
14.1 The section is derived from the ER Monitoring section 🟢 Rows taken from the biomarker table in Monitoring Protocol & Defining Success (ER lines 341–350).
14.2 All measurable/quantifiable biomarkers from the Monitoring section are listed 🟢 All eight ER rows present in ER order, with Optimal Functional Range and Why Measure It? reproduced verbatim; only the Context/Notes column was dropped, as the template has no cell for it.
14.3 [monitoring_cadence] is populated with the monitoring cadence/frequency derived from the ER Monitoring section. It is not left with placeholder text or empty. 🟢 QRS line 693 condenses ER line 339: baseline, 2 and 4 weeks, every 3 months, body composition at 3 and 6 months, full panel annually.

15. Qualitative Assessment

# Description Result Comments
15.1 The section is derived from the ER Monitoring section 🟢 Taken from “Qualitative markers worth tracking alongside the laboratory panel” (ER lines 352–359).
15.2 All subjective/qualitative biomarkers from the Monitoring section are listed 🟢 All six ER bullets present in ER order at QRS lines 702–718.

Issues 13/09/2026 14:26

Pass rate 100.00%. No issues found.