Audit: QRS - Mycoprotein for Health & Longevity

Audit conducted on 22/09/2026 07:57 using AI4L / Opus 5

Iterations

Summary

Items Count
Total 93
Passed 83
Failed 0
N/A 10
Pass Rate 100.00%
  • Total = Passed + Failed + N/A
  • Pass Rate = Passed / (Passed + Failed) × 100
  • N/A items are excluded from the pass rate calculation

1. General Rules

# Description Result Comments
1.1 Every claim, magnitude, label, recommendation, and statement in the QRS is literally supported by content in the source ER. 🟢 Spot-checked every populated span against the ER: protocol values (ER 336–338, 350), time-to-effect (ER 393), benefit/risk tier headings (ER 149–201, 223–269), contraindications (ER 307–312), interactions (ER 289–303), monitoring table (ER 423–434), cadence (ER 421), qualitative items (ER 438–443).
1.2 Where the ER uses cautious phrasing (“not formally studied”, “None documented in human trials to date”, “theoretical concern”, “data are limited”), the QRS uses the same phrasing. 🟢 At-a-glance carries the ER Conclusion’s caution verbatim in substance (“Trials are small and short, with no illness or lifespan data”; ER 467).
1.3 The QRS never strengthens an ER claim (e.g., “not formally studied” → “not required”) or softens one (e.g., “do not use during pregnancy” → “use with caution during pregnancy”). 🟢 Contraindications retain “should avoid” force as decision gates; interactions retain “Caution” force; no directional shift found.
1.4 The QRS does not relabel an ER fact under a different decision category. A “Benefit-Modifying Factor” from ER section is not surfaced as a “Caution”; a “Risk-Modifying Factor” is not surfaced as a “Side Effect”; etc. 🟢 All stop_items come from ER “Populations who should avoid Mycoprotein” (ER 305–312); all caution_items come from the ER interaction bullets (ER 289–303). No Benefit-/Risk-Modifying Factor content appears in the gates.
1.5 PubMed IDs, study citations, expert names, clinical trial identifiers (NCT*), and brand names appear in the QRS only if they appear in the source ER for the same fact. 🟢 The QRS contains no PMIDs, no NCT IDs, no expert names, and no brand names.
1.6 The QRS does not introduce new attributions. 🟢 No attributions present; the ER’s attribution bullet (ER 344) was correctly not carried over.

2. Focus, Tone & Audience

# Description Result Comments
2.1 The QRS follows the tone of the ER, which is determined by the ER’s own language, phrasing, and framing. 🟢 Neutral, declarative, evidence-first register matching the ER throughout.
2.2 The tone of the QRS is simultaneously expert, accessible, objective, and data-driven, but also empowering and encouraging 🟢 Quantified targets and protocol values paired with plain-language framing.
2.3 The QRS reads as a trusted, knowledgeable guide rather than a prescriptive doctor 🟢 Cells state what trials used and what markers show, not what to do.
2.4 The QRS avoids language that implies medical or clinical advice 🟢 No imperatives in any populated span; monitoring cadence is stated descriptively.
2.5 The QRS “presents information” instead of “providing guidance”, “recommending”, or “advising” 🟢 No instances of “recommend”, “advise”, “should”, or “guidance” in populated spans.
2.6 The QRS never addresses “the reader” directly — it presents evidence, not guidance 🟢 Grep for “you/your/yours/yourself” returned zero matches.
2.7 The QRS is written in plain language, avoiding unnecessary medical jargon 🟢 Technical terms retained are ER heading terms carrying the fact itself; no avoidable jargon added.
2.8 Information is presented in a concise and very compact manner 🟢 Tier lines are semicolon-joined ER headings; gate items are single clauses.
2.9 It DOES NOT address the reader directly 🟢 No second-person constructions anywhere in the file.
2.10 The target audience is health- and longevity-oriented adults who are risk-aware, proactive, and actively seeking to optimize health or apply the intervention under review. 🟢 Functional-range monitoring targets and a baseline/4-week urate check assume a proactive optimiser.
2.11 The target audience is willing to employ lifestyle and behavioral changes as well as follow protocols that may be inconvenient, costly, or require effort. 🟢 A 240 g daily substitution split across two meals plus a ten-marker panel presumes that willingness.
2.12 The document is NOT written for the general population, who are unwilling to employ lifestyle and behavioral changes or follow protocols that may be inconvenient, costly, or require effort. 🟢 Apolipoprotein B and fasting-insulin functional targets sit well outside general-population framing.
2.13 Framing, takeaways, and risk/benefit weighting throughout the document reflect this audience, including where an intervention’s signal for the average person differs from its signal for this audience. 🟢 Urate risk is elevated to High tier and given a dedicated gate plus two markers, matching the ER’s weighting for this audience.
2.14 The document’s own voice frames usage in longevity terms, not “anti-aging” (e.g., “anti-aging clinics”, “anti-aging community”, “anti-aging medicine”). Proper names that contain “anti-aging” (e.g., “American Academy of Anti-Aging Medicine”) are quoted verbatim. 🟢 “anti-aging” does not appear; the page title uses “Health & Longevity”.
2.15 The document’s own voice uses formal clinical and scientific terminology, not colloquial or consumer-grade language (e.g., “oral medication” not “pill(s)”; “oral” / “administered orally” not “taken by mouth” / “given by mouth”; “injection” not “shot”; “adverse event” not “bad reaction”). This holds on EVERY surface, including the QRS lede — the stricter plain-language bar (see 2.7) does not license lay phrasing for route of administration. Direct quotes from sources are exempt. 🟢 Lede uses the ER’s own register (“digestive upset”, ER 465); no consumer-grade substitutions for clinical terms.

3. Template Integrity

# Description Result Comments
3.1 The following labels and headings on the QRS are fixed and not modified: Card and section headings: “Protocol”, “Time to effect”, “Benefits”, “Risk & Side Effects”, “Monitoring”, “Qualitative Assessment”; Gate headings: “Contraindications”, “Key Interactions”; Tier labels: “High”, “Medium”, “Low”, “Speculative”; Table column headers in Monitoring: “Marker”, “Target”, “Why” 🟢 Diff against the template shows all fixed headings, gate headings, tier labels, and table headers byte-identical.
3.2 All “…” from the [qrs_template] are present in the the QRS. 🟢 All 34 fixed-name template variables present; the four repeatable names (marker_#name/target/why, qualitative_item#) are correctly expanded to 10 markers and 6 qualitative items.
3.3 Spans that are not addressed in a checklist item are left unchanged 🟢 Full-file diff against the template shows changes confined to variable regions; the website="evidence_review", website="audit", and website="full_review" spans, the CSS block, and the footer disclaimer are untouched.

4. Formatting

# Description Result Comments
4.1 When the source ER section is empty, the QRS uses the ER’s own empty-state phrasing verbatim. Typical phrasings are “None documented in human trials to date” and “Not formally studied” N/A No ER section feeding the QRS is empty; all source sections are populated.
4.2 Where the ER presents a bulleted item as “Label: content”, the QRS uses the ER’s bold label verbatim as the cell or row label. 🟢 action_1/2/3_label reproduce “Standard substitution protocol”, “Acute muscle-support bolus”, “Single versus split dosing” verbatim (ER 336, 338, 350); gate items reproduce the ER interaction bold labels; monitoring row names reproduce the ER biomarker column verbatim.
4.3 Labels are not paraphrased, abbreviated, or invented. 🟢 No invented labels; time-to-effect cell labels derive from the ER “Time to effect” bullet’s own subjects (ER 393), which carries no per-aspect bold labels.
4.4 The QRS DOES NOT use emoji indicators (no 🟩, 🟥, 🟨, etc.). Color and emphasis are conveyed through CSS and bold labels. 🟢 Grep for tier emoji returned zero matches; tiers are conveyed by bold labels and card colour.
4.5 The QRS is designed to render on one A4 page. Any section that has more content in the ER than fits the per-section budget is condensed by the LLM, not extended onto a second page. 🟢 Every section is condensed to its minimum permitted form: tier lines are bare ER headings, gate items single clauses, and the at-a-glance is 58 words. Monitoring (10 rows) and Qualitative (6 items) are at the counts mandated by 14.2 and 15.2.

5. Metadata

# Description Result Comments
5.1 The metadata is placed inside a single HTML comment that is the first element after “<!doctype html>” and before any other comment, head, or body content. 🟢 Lines 2–14 form a single comment immediately after <!doctype html> on line 1.
5.2 Inside that HTML comment the YAML block is delimited by a line “—” opening and a line “—” closing. Text before the opening “—” is permitted but is not parsed as YAML. 🟢 Opening --- at line 3, closing --- at line 13; the descriptive text on line 2 precedes the opening delimiter.
5.3 The metadata is not visible in any rendered view of the QRS and is not surfaced by any other element on the sheet. 🟢 Contained wholly in an HTML comment; no metadata value is echoed in the header or body except the two required subline variables.
5.4 All frontmatter values are trimmed: no leading or trailing whitespace, no surrounding quotes unless the value contains a colon, bracket, or leading special character that requires YAML quoting. 🟢 Only duration: "00:02" is quoted, correctly so because it contains a colon; all other values are bare and trimmed.
5.5 The filename of the source ER is stated as “er_filename: [er_filename]” 🟢 Line 4: er_filename: mycoprotein_2026-0922-0440_Opus_ER.md.
5.6 Version of the QRS.md file used to create the document is stated as “qrs_prompt_version: [Version of QRS.md]” 🟢 Line 5: qrs_prompt_version: 26.9.11, matching the QRS.md version badge.
5.7 Creation date and time of the document is stated as “qrs_creation_date: [YYYY-MMDD-HHMM]” (e.g., 2026-0501-1430) 🟢 Line 6: qrs_creation_date: 2026-0922-0747, conforming to the format.
5.8 The nickname of the AI used to create the document is stated as “qrs_creator_ai_nickname: [qrs_creator_ai_nickname]” 🟢 Line 7: qrs_creator_ai_nickname: Opus.
5.9 The nickname of the AI is just a single word model name without version, etc. (e.g., Opus, Sonnet, Grok, Gemini, ChatGPT) 🟢 “Opus” is a single word with no version or qualifier.
5.10 The full name of the AI used to create the document is stated as “qrs_creator_ai_fullname: [qrs_creator_ai_fullname]” 🟢 Line 8: qrs_creator_ai_fullname: Opus 5.
5.11 The full name of the AI consists of the [qrs_creator_ai_nickname] and the model version number and no additional qualifier (e.g., Opus 4.6, Sonnet 3.2, Grok 4.5, Gemini 3.1, ChatGPT 5.4) 🟢 “Opus 5” is nickname plus version only; no context-window or tier qualifier appended.
5.12 The filename of the document is stated as “qrs_filename: [filename of this document]” 🟢 Line 9 matches the on-disk filename mycoprotein_2026-0922-0440_Opus_QRS.html.
5.13 All frontmatter values are trimmed: no leading or trailing whitespace, no surrounding quotes unless the value contains a colon, bracket, or leading special character that requires YAML quoting. 🟢 Re-verified across all nine keys; no stray whitespace or unnecessary quoting.

6. Page Title & Header

# Description Result Comments
6.1 [page_title] is set to the [canonical_topic] of the ER frontmatter followed by “ - Quick Reference Sheet” (e.g., “Intervention - Quick Reference Sheet”). The [canonical_topic] is HTML-entity-encoded as needed (e.g., &amp; for &) 🟢 Line 22: Mycoprotein for Health &amp; Longevity - Quick Reference Sheet, matching ER frontmatter canonical_topic with & encoded.
6.2 [header_topic] is set to the [canonical_topic] of the ER frontmatter, with HTML entities encoded as needed (e.g., &amp; for &) 🟢 Line 417: Mycoprotein for Health &amp; Longevity.
6.3 [header_subline_date] is set to [qrs_creation_date reformatted as MM/DD/YYYY] 🟢 Line 421: 09/22/2026, the correct reformatting of 2026-0922-0747.
6.4 [header_subline_model] is set to [qrs_creator_ai_fullname] 🟢 Line 425: Opus 5, matching the frontmatter value.
6.5 No additional header content appears: no badge, version stamp, AKA / alternate names line, source-AI attribution, audit date, or QRS variant marker. 🟢 Header holds only the title and the template subline; the ER’s “Also known as” line (ER 32) was correctly omitted.

7. At-A-Glance Section

# Description Result Comments
7.1 [at_a_glance] is dense, execution-oriented summary of the ER Conclusion section 🟢 Leads with the action (“Replacing meat and fish with this fungal food”), then benefits, then offsets, then evidence limits — the ER Conclusion’s own three-paragraph arc (ER 463–467).
7.2 [at_a_glance] is no longer than 60 words 🟢 58 words, counted programmatically.
7.3 Every fact in [at_a_glance] is supported by a distinct passage in the ER. 🟢 Cholesterol/insulin/next-meal intake and muscle from ER 463; uric acid, digestive upset, mould-linked allergy from ER 465; small/short trials and absent illness/lifespan data from ER 467.
7.4 It DOES NOT use acronyms or technical classifications that require specialist knowledge, uses plain-language terms instead 🟢 No acronyms; “LDL”, “postprandial”, and “IgE” are all avoided in favour of “blood cholesterol”, “the insulin rise after a meal”, and “mould-linked allergic reactions”.
7.5 It DOES NOT cite specific trials (names, years, sample sizes, p-values) 🟢 No trial names, years, sample sizes, or p-values appear.
7.6 It DOES NOT cite effect sizes, relative risks, or statistical results 🟢 No numbers of any kind in the span.

8. Contraindications

# Description Result Comments
8.1 The section is derived from the ER Key Interactions & Contraindications section 🟢 All six items trace to “Populations who should avoid Mycoprotein” within that section (ER 305–312).
8.2 [stop_items] represent the Contraindications from the ER 🟢 All six ER avoid-populations are present, none added, none dropped.
8.3 Individual [stop_items] are formatted as <li></li> 🟢 Six <li> elements inside the stop_items span (lines 567–577).
8.4 Items are as concise as possible. No trailing explanations, no elaborations, no mechanistic rationale, no attributions, no citations, no study details. No content after an em-dash, en-dash, or hyphen-dash (e.g., “— dose reduction required”, “— reduced efficacy”) — these trailing clauses are stripped. Just the key fact. 🟢 Leading “Anyone with” stripped from each; ER’s “that use egg white or milk protein as a binder” (ER 311) trimmed; no dashes introduce trailing clauses.
8.5 Parenthetical qualifiers from the ER bullet — time windows, severity classes, threshold values, clinical staging — ARE preserved as part of the item, kept as concise as possible (shortened or trimmed where needed to fit the one-page budget, but never dropped entirely). 🟢 Gout threshold (360 µmol/L, two or more flares in 12 months) and CKD staging (eGFR below 30 mL/min/1.73 m², stage 4 or 5) both preserved; the formulation scopes (“non-vegan”, “contain wheat gluten”) retained.
8.6 When the ER uses ranking notation inside parens (e.g., “>” for severity ordering) that depends on an explanatory phrase to interpret, normalize the items to a plain comma-separated list rather than carrying through the bare symbol. N/A The ER’s contraindication bullets use no ranking notation inside parentheses.
8.7 The section is left empty ONLY IF the ER’s Key Interactions & Contraindications section identifies no population, condition, or scenario for which the intervention should be avoided, deferred, or used only under specialist supervision. 🟢 The ER identifies six such populations and the section is correctly populated rather than empty.
8.8 If the section is left empty, an HTML comment inside the [stop_items] span records the ER basis for the absence, naming or quoting the ER text relied on. E.g. N/A The section is not empty.

9. Key Interactions

# Description Result Comments
9.1 The section is derived from the ER Key Interactions & Contraindications section 🟢 All eight items trace to the ER interaction bullets (ER 289–303).
9.2 [caution_items] represent the Key Interactions from the ER, excluding any that are already listed as Contraindications 🟢 All eight ER interaction bullets are represented; none of them duplicates a contraindication.
9.3 Individual [caution_items] are formatted as <li></li> 🟢 Eight <li> elements inside the caution_items span (lines 585–596).
9.4 Items are as concise as possible. No trailing explanations, no elaborations, no mechanistic rationale, no attributions, no citations, no study details. No content after an em-dash, en-dash, or hyphen-dash (e.g., “— dose reduction required”, “— reduced efficacy”) — these trailing clauses are stripped. Just the key fact. 🟢 Consequence and mitigation sentences stripped from every bullet; the GLP-1 gloss “a gut hormone that signals fullness” and the “over the counter” tag removed; no dash-led trailing clauses.
9.5 Parenthetical qualifiers from the ER bullet — example drug lists, time windows, severity classes, threshold values, clinical staging — ARE preserved as part of the item, kept as concise as possible (shortened or trimmed where needed to fit the one-page budget, but never dropped entirely). 🟢 All four example-drug lists preserved verbatim, and the aspirin dose window “(75–100 mg daily)” retained.
9.6 When the ER uses ranking notation inside parens (e.g., “>” for severity ordering) that depends on an explanatory phrase to interpret, normalize the items to a plain comma-separated list rather than carrying through the bare symbol. N/A The ER’s interaction bullets use no ranking notation inside parentheses.
9.7 The section is left empty ONLY IF the ER’s Key Interactions & Contraindications section identifies no interaction, additive effect, or exposure that changes how the intervention is used. 🟢 The ER identifies eight such interactions and the section is correctly populated rather than empty.
9.8 If the section is left empty, an HTML comment inside the [caution_items] span records the ER basis for the absence, naming or quoting the ER text relied on. E.g. N/A The section is not empty.

10. Protocol

# Description Result Comments
10.1 The section is derived from the ER Protocol section 🟢 All three cells trace to the ER Therapeutic Protocol section (ER 336, 338, 346, 350).
10.2 The three sets of [action] items cover the three most important actionable implementation aspects from the ER Protocol section 🟢 The ER’s best-documented regimen, the acute muscle bolus, and the split-dosing schedule are the three executable decisions; the remaining bullets are attribution, kinetics, and modifier discussion.
10.3 If less that three distinct actionable implementation aspects are mentioned in the ER the unused sets are left empty and made invisible, not filled with placeholder text or empty-state phrasing. N/A The ER supplies more than three actionable aspects, so all three sets are used.
10.4 All used [action_#label], [action#value], [action#_sub] items are filled with meaningful content derived from the ER Protocol section. 🟢 Nine spans populated: labels verbatim from ER bold labels, values “240 g daily” / “70 g” / “Split across two meals”, subs carrying the substitution target, the 31.5 g protein and 2.5 g leucine figures, and the fibre/nucleotide rationale with meal timing.

11. Time to Effect

# Description Result Comments
11.1 The three sets of [time] items cover the three most important time-to-effect aspects from the ER 🟢 Cholesterol, muscle adaptation, and satiety/insulin are exactly the three latencies the ER’s “Time to effect” bullet names (ER 393).
11.2 The sets are picked and ordered by the magnitude of the related benefit 🟢 Order matches the ER’s High-tier benefit ordering: cholesterol (ER 151), muscle (ER 157), then insulin and next-meal intake (ER 163, 169).
11.3 If less that three distinct time-to-effect aspects are mentioned in the ER the unused sets are left empty and made invisible, not filled with placeholder text or empty-state phrasing. N/A The ER names three distinct time-to-effect aspects, so all three sets are used.
11.4 All used [time_#label], [time#value], [time#_sub] items are filled with meaningful content derived from the ER. 🟢 Values “2–4 weeks”, “10 weeks”, “First meal” and subs (eight-week completion, lean-mass parity, single-meal chicken comparator) all trace to ER 393, 161, and 165–173.
11.5 If the ER does not provide any information on time to effect, the section is removed completely from the Protocol Panel N/A The ER provides time-to-effect information (ER 393), so the section is correctly retained.

12. Benefits

# Description Result Comments
12.1 The section is derived from the ER Expected Benefits section 🟢 All eight entries correspond one-to-one with the ER Expected Benefits sub-headings (ER 149–201).
12.2 Key variables are [benefits_high], [benefits_medium], [benefits_low], [benefits_speculative] 🟢 All four spans present and populated (lines 539–557).
12.3 Items are as concise as possible. No explanations, no elaborations, no effect sizes, no qualifiers, no attributions, no citations, no study details, no mechanistic explanations, etc. Just the key fact. 🟢 Each entry is the bare ER sub-heading; the ER’s Magnitude lines, mechanism sentences, and evidence-basis sentences are all excluded.
12.4 Parenthetical content — including effect sizes, sample notes, mechanistic hints, and example studies — is stripped, NOT preserved. 🟢 No parentheses appear in any of the four benefit spans.
12.5 If no items of a specific sub-section (high, medium, low, speculative) are present the respective is set to “display=none”, not filled with “None documented in human trials to date” or similar empty-state phrasing. N/A All four ER tiers contain items, so no span needed hiding.

13. Risks

# Description Result Comments
13.1 The section is derived from the ER Potential Risks & Side Effects section 🟢 All seven entries correspond one-to-one with the ER Potential Risks & Side Effects sub-headings (ER 223–269).
13.2 Key variables are [risks_high], [risks_medium], [risks_low], [risks_speculative] 🟢 All four spans present and populated (lines 608–622).
13.3 Items are as concise as possible. No explanations, no elaborations, no effect sizes, no qualifiers, no attributions, no citations, no study details, no mechanistic explanations, etc. Just the key fact. 🟢 Each entry is the bare ER sub-heading; the registry counts, percentages, and CSPI attribution in the ER body are all excluded.
13.4 Parenthetical content — including frequencies, severity grades, sample notes, mechanistic hints, and example studies — is stripped, NOT preserved. 🟢 No parentheses appear in any of the four risk spans.
13.5 If no items of a specific sub-section (high, medium, low, speculative) are present the respective is set to “display=none”, not filled with “None documented in human trials to date” or similar empty-state phrasing. N/A All four ER tiers contain items, so no span needed hiding.

14. Monitoring

# Description Result Comments
14.1 The section is derived from the ER Monitoring section 🟢 Table rows and cadence both trace to the ER Monitoring Protocol & Defining Success section (ER 419–434).
14.2 All measurable/quantifiable biomarkers from the Monitoring section are listed 🟢 All ten ER table biomarkers present in ER order, with Optimal Functional Range and “Why Measure It?” reproduced verbatim into the Target and Why columns.
14.3 [monitoring_cadence] is populated with the monitoring cadence/frequency derived from the ER Monitoring section. It is not left with placeholder text or empty. 🟢 Lines 774–777 reproduce the ER’s ongoing-monitoring schedule (ER 421): urate at 4 weeks, lipids and apolipoprotein B at 8–12 weeks, then 6–12 monthly; vitamin B12 and ferritin at 6 months then annually.

15. Qualitative Assessment

# Description Result Comments
15.1 The section is derived from the ER Monitoring section 🟢 All six items come from the ER’s “Qualitative markers worth tracking alongside the laboratory values” list (ER 436–443).
15.2 All subjective/qualitative biomarkers from the Monitoring section are listed 🟢 All six ER qualitative markers present in ER order and verbatim: gastrointestinal comfort, allergic-reaction signs, fullness/time to next hunger, joint pain, training recovery, and afternoon energy stability.

Issues 22/09/2026 07:57

Pass rate 100.00%. No issues found.

Issues 22/09/2026 07:52

  1. 11.4 — Time-to-effect subs restate values: time_2_sub (line 514) and time_3_sub (lines 525–527) repeat their own label and value verbatim (“Muscle adaptation / 10 weeks / Muscle adaptations took ten weeks of training”), contributing no information.
  2. 12.3 — Conflicted qualifier in benefits: benefits_high (line 541) keeps “(conflicted)” after “blunted postprandial insulin and glucose response”, a redundant evidence-strength qualifier.
  3. 13.3 — Conflicted qualifier in risks: risks_low (line 615) keeps “(conflicted)” after “no reliable triglyceride benefit”, a redundant evidence-strength qualifier.

Fixes 22/09/2026 07:52

  1. 11.4 — Time-to-effect subs restate values: Replaced time_2_sub “Muscle adaptations took ten weeks of training” with “Lean mass gain matched an omnivorous diet over the training block”, and time_3_sub “Satiety and the blunted insulin response appear at the first meal” with “Both measured in single-meal trials, against an equal-energy chicken meal”.
  2. 12.3 / 13.3 — Conflicted qualifier stripped: Removed the redundant “(conflicted)” qualifier from benefits_high after “blunted postprandial insulin and glucose response” and from risks_low after “no reliable triglyceride benefit”.