Audit: QRS - PAI-1 Inhibitors for Health & Longevity

Audit conducted on 01/09/2026 17:15 using AI4L / Opus 5

Iterations

Summary

Items Count
Total 93
Passed 85
Failed 0
N/A 8
Pass Rate 100.00%
  • Total = Passed + Failed + N/A
  • Pass Rate = Passed / (Passed + Failed) × 100
  • N/A items are excluded from the pass rate calculation

1. General Rules

# Description Result Comments
1.1 Every claim, magnitude, label, recommendation, and statement in the QRS is literally supported by content in the source ER. 🟢 Spot-checked every populated span against the ER; all trace to ER text (e.g. protocol cells to ER lines 321/323/331, time cells to ER line 378, monitoring rows to ER table lines 406-415).
1.2 Where the ER uses cautious phrasing (“not formally studied”, “None documented in human trials to date”, “theoretical concern”, “data are limited”), the QRS uses the same phrasing. 🟢 Cautious framing preserved: ‘No approved protocol exists’, ‘Registered trials only’, ‘all without comparison groups’, ‘None is approved anywhere’.
1.3 The QRS never strengthens an ER claim (e.g., “not formally studied” → “not required”) or softens one (e.g., “do not use during pregnancy” → “use with caution during pregnancy”). 🟢 No strengthening or softening detected; contraindication thresholds and severity classes carried across unchanged.
1.4 The QRS does not relabel an ER fact under a different decision category. A “Benefit-Modifying Factor” from ER section is not surfaced as a “Caution”; a “Risk-Modifying Factor” is not surfaced as a “Side Effect”; etc. 🟢 Benefit- and Risk-Modifying Factors are not surfaced anywhere; gates draw only from the ER Key Interactions & Contraindications section.
1.5 PubMed IDs, study citations, expert names, clinical trial identifiers (NCT*), and brand names appear in the QRS only if they appear in the source ER for the same fact. 🟢 Only brand/compound name used is TM5614 (action_2_sub), which the ER names for the same regimen fact. No PMIDs, NCT IDs, author names or years appear.
1.6 The QRS does not introduce new attributions. 🟢 No attributions introduced; no expert, institution, or sponsor is named anywhere in the QRS.

2. Focus, Tone & Audience

# Description Result Comments
2.1 The QRS follows the tone of the ER, which is determined by the ER’s own language, phrasing, and framing. 🟢 Matches the ER’s sober, evidence-limited register throughout.
2.2 The tone of the QRS is simultaneously expert, accessible, objective, and data-driven, but also empowering and encouraging 🟢 Expert, objective and data-driven; the restrained framing tracks the ER’s own evidence base rather than overselling.
2.3 The QRS reads as a trusted, knowledgeable guide rather than a prescriptive doctor 🟢 Presents trial-derived facts; no directive voice.
2.4 The QRS avoids language that implies medical or clinical advice 🟢 No advice language; protocol cells are framed as what investigators did inside registered trials.
2.5 The QRS “presents information” instead of “providing guidance”, “recommending”, or “advising” 🟢 No ‘recommend’, ‘advise’ or ‘should’ constructions in the document’s own voice.
2.6 The QRS never addresses “the reader” directly — it presents evidence, not guidance 🟢 No second-person address anywhere in the file.
2.7 The QRS is written in plain language, avoiding unnecessary medical jargon 🟢 Plain language used where possible; residual technical terms (Child-Pugh, HOMA-IR, eGFR) are ER-sourced biomarker and gate names that cannot be dropped without losing the decision content.
2.8 Information is presented in a concise and very compact manner 🟢 Gate and tier items are reduced to noun phrases; rationale, mechanism and citations stripped.
2.9 It DOES NOT address the reader directly 🟢 Confirmed — no direct address.
2.10 The target audience is health- and longevity-oriented adults who are risk-aware, proactive, and actively seeking to optimize health or apply the intervention under review. 🟢 Framing suits risk-aware self-directed adults: access route, dose, timing and monitoring panel are foregrounded.
2.11 The target audience is willing to employ lifestyle and behavioral changes as well as follow protocols that may be inconvenient, costly, or require effort. 🟢 Monitoring cadence and 10-marker panel assume willingness to follow an effortful protocol.
2.12 The document is NOT written for the general population, who are unwilling to employ lifestyle and behavioral changes or follow protocols that may be inconvenient, costly, or require effort. 🟢 Not pitched at the general population; assumes biomarker literacy.
2.13 Framing, takeaways, and risk/benefit weighting throughout the document reflect this audience, including where an intervention’s signal for the average person differs from its signal for this audience. 🟢 Correctly signals that no legitimate route exists for a healthy adult, which is the decisive point for this audience.
2.14 The document’s own voice frames usage in longevity terms, not “anti-aging” (e.g., “anti-aging clinics”, “anti-aging community”, “anti-aging medicine”). Proper names that contain “anti-aging” (e.g., “American Academy of Anti-Aging Medicine”) are quoted verbatim. 🟢 No occurrence of ‘anti-aging’ or ‘antiaging’; ‘Longevity’ used in the title and At-A-Glance.
2.15 The document’s own voice uses formal clinical and scientific terminology, not colloquial or consumer-grade language (e.g., “oral medication” not “pill(s)”; “injection” not “shot”; “adverse event” not “bad reaction”). Direct quotes from sources are exempt. 🟢 ‘oral medications’, ‘adverse’-register terminology used; no colloquialisms in the document’s own voice.

3. Template Integrity

# Description Result Comments
3.1 The following labels and headings on the QRS are fixed and not modified: Card and section headings: “Protocol”, “Time to effect”, “Benefits”, “Risk & Side Effects”, “Monitoring”, “Qualitative Assessment”; Gate headings: “Contraindications”, “Key Interactions”; Tier labels: “High”, “Medium”, “Low”, “Speculative”; Table column headers in Monitoring: “Marker”, “Target”, “Why” 🟢 All fixed headings match [qrs_template] byte-for-byte: ‘Protocol’, ‘Time to effect’, ‘Benefits’, ‘Risk & Side Effects’, ‘Monitoring’, ‘Qualitative Assessment’, ‘Contraindications’, ‘Key Interactions’, tier labels, and ‘Marker’/’Target’/’Why’.
3.2 All “<span data-qrs-var=”NAME”>…</span>” from the [qrs_template] are present in the the QRS. 🟢 All 34 distinct template spans present; repeatable marker_#* and qualitative_item# rows instantiated 10x and 5x respectively.
3.3 Spans that are not addressed in a checklist item are left unchanged 🟢 Head, CSS and the three elements are identical to the template; only checklist-addressed spans differ.

4. Formatting

# Description Result Comments
4.1 When the source ER section is empty, the QRS uses the ER’s own empty-state phrasing verbatim. Typical phrasings are “None documented in human trials to date” and “Not formally studied” N/A No source ER section is empty. The ER’s High tiers carry explanatory text (‘No benefit reaches High…’, ‘No adverse effect reaches High…’), which items 12.5/13.5 govern instead.
4.2 Where the ER presents a bulleted item as “Label: content”, the QRS uses the ER’s bold label verbatim as the cell or row label. 🟢 Protocol labels are the ER’s bold labels verbatim (‘No approved protocol exists’, ‘Standard trial regimen’, ‘Best time of day’); monitoring marker names and ‘Why’ cells are verbatim from the ER table.
4.3 Labels are not paraphrased, abbreviated, or invented. 🟢 No paraphrased or invented labels; time-to-effect labels (‘Molecular response’, ‘Tumour response’, ‘Oxygen requirement’) are taken verbatim from ER line 378.
4.4 The QRS DOES NOT use emoji indicators (no 🟩, 🟥, 🟨, etc.). Color and emphasis are conveyed through CSS and bold labels. 🟢 No emoji present anywhere in the file; the ER’s tier squares and the two ‘⚠️ Conflicted’ markers were correctly dropped.
4.5 The QRS is designed to render on one A4 page. Any section that has more content in the ER than fits the per-section budget is condensed by the LLM, not extended onto a second page. 🟢 Condensation applied as required: benefit/risk tiers reduced to ER headings only, gate glosses and mechanistic rationale stripped, marker targets trimmed. Remaining volume (11 contraindications, 10 markers) is mandated by items 8.2 and 14.2.

5. Metadata

# Description Result Comments
5.1 The metadata is placed inside a single HTML comment that is the first element after “<!doctype html>” and before any other comment, head, or body content. 🟢 Metadata comment opens at line 2, immediately after <!doctype html>, before any other content.
5.2 Inside that HTML comment the YAML block is delimited by a line “—” opening and a line “—” closing. Text before the opening “—” is permitted but is not parsed as YAML. 🟢 YAML delimited by ‘—’ at lines 3 and 13; the preceding ‘QRS — Metadata’ text sits outside the block, which is permitted.
5.3 The metadata is not visible in any rendered view of the QRS and is not surfaced by any other element on the sheet. 🟢 Enclosed in an HTML comment; not surfaced by any element on the sheet.
5.4 All frontmatter values are trimmed: no leading or trailing whitespace, no surrounding quotes unless the value contains a colon, bracket, or leading special character that requires YAML quoting. 🟢 All values trimmed; only ‘duration: “00:04”’ is quoted, which is required because the value contains a colon.
5.5 The filename of the source ER is stated as “er_filename: [er_filename]” 🟢 er_filename: pai_1_inhibitors_2026-0901-1524_Opus_ER.md
5.6 Version of the QRS.md file used to create the document is stated as “qrs_prompt_version: [Version of QRS.md]” 🟢 qrs_prompt_version: 26.7.02, matching the version badge of QRS.md.
5.7 Creation date and time of the document is stated as “qrs_creation_date: [YYYY-MMDD-HHMM]” (e.g., 2026-0501-1430) 🟢 qrs_creation_date: 2026-0901-1707 — correct YYYY-MMDD-HHMM form.
5.8 The nickname of the AI used to create the document is stated as “qrs_creator_ai_nickname: [qrs_creator_ai_nickname]” 🟢 qrs_creator_ai_nickname: Opus
5.9 The nickname of the AI is just a single word model name without version, etc. (e.g., Opus, Sonnet, Grok, Gemini, ChatGPT) 🟢 Single word, no version or qualifier.
5.10 The full name of the AI used to create the document is stated as “qrs_creator_ai_fullname: [qrs_creator_ai_fullname]” 🟢 qrs_creator_ai_fullname: Opus 5
5.11 The full name of the AI consists of the [qrs_creator_ai_nickname] and the model version number and no additional qualifier (e.g., Opus 4.6, Sonnet 3.2, Grok 4.5, Gemini 3.1, ChatGPT 5.4) 🟢 Nickname plus version number only; no additional qualifier.
5.12 The filename of the document is stated as “qrs_filename: [filename of this document]” 🟢 qrs_filename: pai_1_inhibitors_2026-0901-1524_Opus_QRS.html — matches the file on disk.
5.13 All frontmatter values are trimmed: no leading or trailing whitespace, no surrounding quotes unless the value contains a colon, bracket, or leading special character that requires YAML quoting. 🟢 Confirmed; no stray whitespace or unnecessary quoting.

6. Page Title & Header

# Description Result Comments
6.1 [page_title] is set to the [canonical_topic] of the ER frontmatter followed by “ - Quick Reference Sheet” (e.g., “Intervention - Quick Reference Sheet”). The [canonical_topic] is HTML-entity-encoded as needed (e.g., &amp; for &) 🟢 PAI-1 Inhibitors for Health & Longevity - Quick Reference Sheet — matches the ER canonical_topic with ‘&’ entity-encoded.
6.2 [header_topic] is set to the [canonical_topic] of the ER frontmatter, with HTML entities encoded as needed (e.g., &amp; for &) 🟢 header_topic is ‘PAI-1 Inhibitors for Health & Longevity’, entity-encoded.
6.3 [header_subline_date] is set to [qrs_creation_date reformatted as MM/DD/YYYY] 🟢 09/01/2026, correctly derived from qrs_creation_date 2026-0901-1707.
6.4 [header_subline_model] is set to [qrs_creator_ai_fullname] 🟢 header_subline_model is ‘Opus 5’, matching qrs_creator_ai_fullname.
6.5 No additional header content appears: no badge, version stamp, AKA / alternate names line, source-AI attribution, audit date, or QRS variant marker. 🟢 Header is byte-identical to the template apart from the two variable spans; no badge, AKA line, or audit stamp added.

7. At-A-Glance Section

# Description Result Comments
7.1 [at_a_glance] is dense, execution-oriented summary of the ER Conclusion section 🟢 Condenses the ER Conclusion (lines 451-457) into the access-decisive points: mechanism, the genetic basis of the longevity claim, the narrowness of human data, and the absence of approval.
7.2 [at_a_glance] is no longer than 60 words 🟢 57 words — under the 60-word limit.
7.3 Every fact in [at_a_glance] is supported by a distinct passage in the ER. 🟢 Each clause maps to a distinct Conclusion passage; ‘None is approved anywhere’ is additionally supported by ER line 365.
7.4 It DOES NOT use acronyms or technical classifications that require specialist knowledge, uses plain-language terms instead 🟢 No acronyms or specialist classifications; ‘PAI-1’, ‘SERPINE1’, ‘fibrinolysis’ and ‘senescence’ are all avoided in favour of plain paraphrase.
7.5 It DOES NOT cite specific trials (names, years, sample sizes, p-values) 🟢 No trial names, years, sample sizes or p-values.
7.6 It DOES NOT cite effect sizes, relative risks, or statistical results 🟢 No effect sizes, relative risks, or statistical results.

8. Contraindications

# Description Result Comments
8.1 The section is derived from the ER Key Interactions & Contraindications section 🟢 Drawn from the ER Key Interactions & Contraindications section (lines 270-301).
8.2 [stop_items] represent the Contraindications from the ER 🟢 All 10 items of the ER ‘Populations who should avoid’ list are present, plus thrombolytic agents, which the ER’s interaction bullet labels an ‘Absolute contraindication’ (line 276).
8.3 Individual [stop_items] are formatted as <li></li> 🟢 Each item is its own <li> inside the [stop_items] span.
8.4 Items are as concise as possible. No trailing explanations, no elaborations, no mechanistic rationale, no attributions, no citations, no study details. No content after an em-dash, en-dash, or hyphen-dash (e.g., “— dose reduction required”, “— reduced efficacy”) — these trailing clauses are stripped. Just the key fact. 🟢 Explanatory glosses stripped (‘a tangle of abnormal blood vessels’, ‘a ballooned artery wall’, ‘clots in the veins or lungs’); no trailing rationale, citations, or dash clauses remain.
8.5 Parenthetical qualifiers from the ER bullet — time windows, severity classes, threshold values, clinical staging — ARE preserved as part of the item, kept as concise as possible (shortened or trimmed where needed to fit the one-page budget, but never dropped entirely). 🟢 Thresholds and windows preserved: ‘below 50 × 10⁹/L or haematocrit below 30%’, ‘within 90 days’, ‘(systolic above 200 mmHg beyond 12 hours)’, ‘Child-Pugh Class B or C (moderate to severe)’.
8.6 When the ER uses ranking notation inside parens (e.g., “>” for severity ordering) that depends on an explanatory phrase to interpret, normalize the items to a plain comma-separated list rather than carrying through the bare symbol. N/A The ER uses no ranking notation inside parentheses in this section.
8.7 The section is left empty ONLY IF the ER’s Key Interactions & Contraindications section identifies no population, condition, or scenario for which the intervention should be avoided, deferred, or used only under specialist supervision. 🟢 The section is populated; the ER does identify populations that should avoid the intervention.
8.8 If the section is left empty, an HTML comment inside the [stop_items] span records the ER basis for the absence, naming or quoting the ER text relied on. E.g. <!– empty: ER names no population that should avoid the intervention –> N/A The section is not empty.

9. Key Interactions

# Description Result Comments
9.1 The section is derived from the ER Key Interactions & Contraindications section 🟢 Drawn from the ER Key Interactions & Contraindications section (lines 272-288).
9.2 [caution_items] represent the Key Interactions from the ER, excluding any that are already listed as Contraindications 🟢 Seven of the ER’s nine interaction bullets are carried. Thrombolytics and anticoagulants are correctly omitted because both are already captured in [stop_items] (‘Thrombolytic agents’, ‘Concurrent full-dose anticoagulation’).
9.3 Individual [caution_items] are formatted as <li></li> 🟢 Each item is its own <li> inside the [caution_items] span.
9.4 Items are as concise as possible. No trailing explanations, no elaborations, no mechanistic rationale, no attributions, no citations, no study details. No content after an em-dash, en-dash, or hyphen-dash (e.g., “— dose reduction required”, “— reduced efficacy”) — these trailing clauses are stripped. Just the key fact. 🟢 Mechanistic glosses and mitigation clauses stripped (e.g. ‘which stop platelets clumping’, ‘which release brakes on the immune system’); no dash-trailing content remains.
9.5 Parenthetical qualifiers from the ER bullet — example drug lists, time windows, severity classes, threshold values, clinical staging — ARE preserved as part of the item, kept as concise as possible (shortened or trimmed where needed to fit the one-page budget, but never dropped entirely). 🟢 Named example drug lists preserved in full for all seven items, including the nine-item supplement list and the four named procedures.
9.6 When the ER uses ranking notation inside parens (e.g., “>” for severity ordering) that depends on an explanatory phrase to interpret, normalize the items to a plain comma-separated list rather than carrying through the bare symbol. N/A The ER uses no ranking notation inside parentheses in this section.
9.7 The section is left empty ONLY IF the ER’s Key Interactions & Contraindications section identifies no interaction, additive effect, or exposure that changes how the intervention is used. 🟢 The section is populated; the ER does identify interactions that change how the intervention is used.
9.8 If the section is left empty, an HTML comment inside the [caution_items] span records the ER basis for the absence, naming or quoting the ER text relied on. E.g. <!– empty: ER names no interaction that changes how the intervention is used –> N/A The section is not empty.

10. Protocol

# Description Result Comments
10.1 The section is derived from the ER Protocol section 🟢 Drawn from the ER Therapeutic Protocol section (lines 319-345).
10.2 The three sets of [action] items cover the three most important actionable implementation aspects from the ER Protocol section 🟢 The three chosen aspects are the access gate, the dose regimen, and the dosing time — the only directly actionable bullets in the ER’s protocol list; the remainder are ‘no data established’ entries.
10.3 If less that three distinct actionable implementation aspects are mentioned in the ER the unused sets are left empty and made invisible, not filled with placeholder text or empty-state phrasing. N/A The ER names more than three actionable implementation aspects, so no set is left unused.
10.4 All used [action_#label], [action#value], [action#_sub] items are filled with meaningful content derived from the ER Protocol section. 🟢 All nine action fields carry substantive ER-derived content; no placeholders remain.

11. Time to Effect

# Description Result Comments
11.1 The three sets of [time] items cover the three most important time-to-effect aspects from the ER 🟢 All three time-to-effect aspects the ER states (ER line 378) are represented.
11.2 The sets are picked and ordered by the magnitude of the related benefit 🟢 Ordered by benefit tier: molecular response (Medium), tumour response (Medium), oxygen requirement (Low) — matching the ER’s own ordering within tiers.
11.3 If less that three distinct time-to-effect aspects are mentioned in the ER the unused sets are left empty and made invisible, not filled with placeholder text or empty-state phrasing. N/A The ER provides three distinct time-to-effect aspects, so no set is left unused.
11.4 All used [time_#label], [time#value], [time#_sub] items are filled with meaningful content derived from the ER. 🟢 All nine time fields populated from ER line 378; time_3_sub also carries the ER’s caveat that no longevity timeline exists.
11.5 If the ER does not provide any information on time to effect, the section is removed completely from the Protocol Panel N/A The ER does provide time-to-effect information, so the section is correctly retained.

12. Benefits

# Description Result Comments
12.1 The section is derived from the ER Expected Benefits section 🟢 Drawn from the ER Expected Benefits section (lines 130-198).
12.2 Key variables are [benefits_high], [benefits_medium], [benefits_low], [benefits_speculative] 🟢 All four benefit spans present and correctly named.
12.3 Items are as concise as possible. No explanations, no elaborations, no effect sizes, no qualifiers, no attributions, no citations, no study details, no mechanistic explanations, etc. Just the key fact. 🟢 Items reduced to the ER’s own subsection headings; no magnitudes, confidence intervals, mechanisms, or citations carried over.
12.4 Parenthetical content — including effect sizes, sample notes, mechanistic hints, and example studies — is stripped, NOT preserved. 🟢 All parenthetical content stripped; no ‘(95% confidence interval …)’ or sample notes appear.
12.5 If no items of a specific sub-section (high, medium, low, speculative) are present the respective <SPAN> is set to “display=none”, not filled with “None documented in human trials to date” or similar empty-state phrasing. 🟢 [benefits_high] is emptied and set to style=”display: none”; the ER’s ‘No benefit reaches High’ sentence is correctly not reproduced.

13. Risks

# Description Result Comments
13.1 The section is derived from the ER Potential Risks & Side Effects section 🟢 Drawn from the ER Potential Risks & Side Effects section (lines 214-254).
13.2 Key variables are [risks_high], [risks_medium], [risks_low], [risks_speculative] 🟢 All four risk spans present and correctly named.
13.3 Items are as concise as possible. No explanations, no elaborations, no effect sizes, no qualifiers, no attributions, no citations, no study details, no mechanistic explanations, etc. Just the key fact. 🟢 Items reduced to the ER’s own subsection headings; the ‘⚠️ Conflicted’ marker and all mechanism text stripped.
13.4 Parenthetical content — including frequencies, severity grades, sample notes, mechanistic hints, and example studies — is stripped, NOT preserved. 🟢 No frequencies or severity grades carried over; the ER’s ‘2 of 34 patients (5.9%)’ and ‘grade 3 or higher’ are absent.
13.5 If no items of a specific sub-section (high, medium, low, speculative) are present the respective <SPAN> is set to “display=none”, not filled with “None documented in human trials to date” or similar empty-state phrasing. 🟢 [risks_high] is emptied and set to style=”display: none”; the ER’s ‘No adverse effect reaches High’ sentence is correctly not reproduced.

14. Monitoring

# Description Result Comments
14.1 The section is derived from the ER Monitoring section 🟢 Drawn from the ER Monitoring Protocol & Defining Success section (lines 400-415).
14.2 All measurable/quantifiable biomarkers from the Monitoring section are listed 🟢 All 10 ER biomarker rows are present in order, with targets and ‘Why’ cells matching the ER table.
14.3 [monitoring_cadence] is populated with the monitoring cadence/frequency derived from the ER Monitoring section. It is not left with placeholder text or empty. 🟢 Cadence populated from ER line 402: ‘Baseline, one week, four weeks, eight weeks, then every three months … tightening to weekly …’.

15. Qualitative Assessment

# Description Result Comments
15.1 The section is derived from the ER Monitoring section 🟢 Drawn from the qualitative marker list at the end of the ER Monitoring Protocol & Defining Success section (lines 419-423).
15.2 All subjective/qualitative biomarkers from the Monitoring section are listed 🟢 All five ER qualitative markers are reproduced verbatim.

Issues 01/09/2026 17:15

Pass rate 100.00%. No issues found.